Self-fertilization in a gonochoristic fish yellow catfish  

作　　者：Yue Yu Ziyi Lu Min Chen Ya Liu Yang Ding Chenhao Hu Zhigang Shen 

机构地区：[1]College of Fisheries,Key Lab of Freshwater Animal Breeding,Ministry of Agriculture and Rural Affairs,Wuhan,430070,China [2]Key Lab of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Wuhan,430070,China [3]Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province,Wuhan,430070,China [4]Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt of Ministry of Education,Huazhong Agricultural University,Wuhan,430070,China [5]Hubei Huangyouyuan Fishery Development Limited Company,Wuhan,430070,China

出　　处：《Aquaculture and Fisheries》2024年第2期234-244,共11页渔业学报（英文）

基　　金：supported by the National Natural Science Foundation of China(32273122);Major Science and Technology project of Hubei Province(2023BBA001);Fundamental Research Funds for the Central Universities(2662021SCPY001);the Natural Science Foundation of Hubei Province(2018CFB215).

摘　　要：Sex control techniques are in great demand for aquaculture because of the huge benefits of mono-sex production.In a previous study on yellow catfish(Tachysurus fulvidraco),intersex individuals were produced by administrating 17α-methyltestosterone(MT)or aromatase inhibitor letrozole(LZ).The ovarian and testicular portions of intersexual gonads developed well and were completely separated,arousing our interest in producing self-fertilizable fish and clone-like fish populations.In this study,yellow catfish fry were treated with a combination of MT and LZ at a low dose(5 mg kg^(-1)MT,300 mg kg^(-1)LZ)at a low water temperature(27.0℃,mixed sex-producing temperature,LL group)and a high temperature(33.5◦C,male-producing temperature,HL group),and with a high dose(10 mg kg^(-1) MT,600 mg kg^(-1) LZ)at a low water temperature(LH group),during 12-65 days post-hatching,the critical period of sex differentiation.Both XX and XY intersex were produced with different percentages at the same time through co-treatment with MT and LZ,with or without the help of male-producing temperature.The highest percentage of XY intersex(53%in XY,36%in XX and XY)and XX intersex(47%in XX,25%in XX and XY)was produced in LH and HL groups,respectively.Results of gonadal morphology,histology,and sperm dynamic analysis indicate that some XX and XY intersex individuals were capable of self-fertilization through artificial insemination.The reproductive performance of these intersex individuals was then investigated by injecting spawning agents,dissecting gonads,and artificially fertilizing eggs with their own sperm.The XX,XY,and YY genotypic individuals were produced by the self-fertilization of XY intersex individuals.The present study successfully produced high percentages of XX and XY intersex and conducted self-fertilization by mating the eggs and sperm of a single intersex individual.Self-fertilization in fish will show great potential in aquaculture genetics and breeding,for example,generating highly homozygous(or"clonal")genetic material,p

关 键 词：Sex differentiation SELF-FERTILIZATION Sex reversal Mono-sex CLONE 

分 类 号：Q955[生物学—动物学]