Differences in leaf cuticular wax induced by whole-genome duplication in autotetraploid sour jujube  

作　　者：Meng Li Chenxing Zhang Lu Hou Xinru Liu Hanqing Zhao Xiaoming Pang Wenhao Bo Yingyue Li 

机构地区：[1]National Engineering Research Center of Tree Breeding and Ecological Restoration,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China [2]Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China [3]The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration,Beijing Forestry University,Beijing 100083,China

出　　处：《Horticultural Plant Journal》2024年第1期66-76,共11页园艺学报（英文版）

基　　金：supported by grants from the Fundamental Research Funds for the Central Universities(Grant No.2021JD02);the National Key Research and Development Program of China(Grant No.2018YFD1000607)。

摘　　要：Drought-resistant plants exhibit strong water retention capability.In this regard,the autotetraploid sour jujube leaves exhibit better water retention than diploid leaves.Morphological comparisons and physiological comparisons of diploid and autotetraploid leaves showed that the autotetraploid leaves had thicker leaf cuticles and more leaf wax accumulation than the diploid leaves,which could reduce cuticle permeability and improve the drought tolerance of leaves.In this study,the cuticular wax crystalloids on the adaxial and abaxial sides of young and mature jujube leaves were observed in the two ploidy types,and unique cuticular wax crystalloids covering a large area of the cuticle on autotetraploid sour jujube leaves may provide an advantage in reducing leaf non-stomata transpiration and improving plant drought tolerance.Based on the transcriptome,115 differentially expressed genes between diploids and autotetraploids were further analyzed and found to be involved in the accumulation of cuticular wax components,including terpenoids,fatty acids,and lipids,as well as ABC transporter and wax biosynthetic process.Finally,14 genes differentially expressed between glossy autotetraploid leaves and nonglossy diploid leaves,such as LOC107414787,LOC107411574 and LOC107413721,were screened as candidate genes by qRT-PCR analysis.This findings provided insights into how polyploidization improved drought tolerance.

关 键 词：AUTOTETRAPLOID JUJUBE Drought tolerance Cuticular wax RNA-seq 

分 类 号：S665.1[农业科学—果树学]