FaSnRK1a mediates salicylic acid pathways to enhance strawberry resistance to Botrytis cinerea  

作　　者：Jingjing Luo Wenying Yu Yuansong Xiao Yafei Zhang Futian Peng 

机构地区：[1]State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China

出　　处：《Horticultural Plant Journal》2024年第1期131-144,共14页园艺学报（英文版）

基　　金：supported by National Natural Science Foundation of China(Grant Nos.31672099,31801812);the National Modern Agroindustry Technology Research System Fund(Grant No.CARS-30-2-02)。

摘　　要：Strawberry is a major fruit crop worldwide because its nutritional and health benefits to human health,but its productivity is limited by Botrytis cinerea.Sucrose nonfermentation 1-related protein kinase 1(SnRK1)has a defense function against pathogens,but the function of SnRK1 in the defense response to B.cinerea in plants is still unclear.In this study,FaSnRK1a-OE and RNAi fruits were constructed and then inoculated with B.cinerea.The result reveals a positive role of Fa SnRK1a in the regulation of resistance to gray mold.FaSnRK1a affects SA content by regulating FaPAL1 and FaPAL2 expressions.The genes related to the SA signaling pathway(FaTGA1 and FaTGA2.1)were significantly increased/decreased in FaSnRK1a-OE or FaSnRK1a-RNAi fruit,respectively.FaSnRK1a interacted with the FaWRKY33.2 protein and negatively regulated FaWRKY33.2 expression,and FaWRKY33.2 acts as a repressor of disease resistance to B.cinerea.Finally,FaSnRK1a regulates the expression of six PR genes and the activities of antioxidant enzymes to boost defense response after B.cinerea inoculation.Our findings showed that FaSnRK1a increases the resistance of strawberry fruit to B.cinerea via SA signaling pathway and interaction with the FaWRKY33.2 transcription factor.

关 键 词：STRAWBERRY B.cinerea SnRK1 Salicylic acid WRKY transcription factor 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]