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作 者:Jia Liu Junjie Cui Jichi Dong Jian Zhong Chunfeng Zhong Fanchong Yuan Wendong Guan Fang Hu Jiaowen Cheng Kailin Hu
机构地区:[1]College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642,China [2]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China),Ministry of Agriculture and Rural Affairs,Guangzhou,Guangdong 510642,China [3]Guangdong Vegetables Engineering Research Center,Guangzhou,Guangdong 510642,China [4]Department of Horticulture,Foshan University,Foshan,Guangdong 528225,China [5]Henry Fok School of Biology and Agricultural,Shaoguan University,Shaoguan,Guangdong 512023,China
出 处:《Horticultural Plant Journal》2024年第1期171-180,共10页园艺学报(英文版)
基 金:supported by the Science and Technology Planning Project of Guangdong Province(Grants Nos.2022 B0202160015 and 2019A050520002);the Seed Industry Revitalization Project of Special Funds for Provincial Rural Revitalization Strategy(Grant No.2022-NPY-00-027);the Guangzhou Science and Technology Plan Projects(Grants Nos.202002020086,202102020800 and 202206010170);the Guangzhou Basic and Applied Basic Research Project(Grant No.SL2023A04J01673)。
摘 要:Fruit wart is an important appearance trait influencing consumer preferences of bitter gourd(Momordica charantia L.).The molecular genetic mechanisms underlying fruit wart formation in bitter gourd are largely unknown.In this study,genetic analysis based on four generations showed that fruit wart formation in bitter gourd was controlled by a single dominant locus named as Fwa.The Fwa locus was initially mapped into a 4.82 Mb region on pseudochromosome 4 by BSA-seq analysis and subsequently narrowed down to a 286.30 kb region by linkage analysis.A large F2population consisting of 2360 individuals was used to screen recombinants,and the Fwa locus was finally fine mapped into a 22.70 kb region harboring four protein-coding genes through recombination analysis.MC04g1399,encoding an epidermal patterning factor 2-like protein,was proposed as the best candidate gene for Fwa via sequence variation and expression analysis.In addition,a 1-bp insertion and deletion(InDel)variation within MC04g1399 was converted to a cleaved amplified polymorphic sequence(CAPS)marker that could precisely distinguish between the warty and non-warty types with an accuracy rate of 100%among a wide panel of 126 bitter gourd germplasm resources.Our results not only provide a scientific basis for deciphering the molecular mechanisms underlying fruit wart formation but also provide a powerful tool for efficient genetic improvement of fruit wart via marker-assisted selection.
关 键 词:Bitter gourd Fruit wart Bulk segregant analysis FINE-MAPPING Candidate gene
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