CmMYB3-like negatively regulates anthocyanin biosynthesis and flower color formation during the post-flowering stage in Chrysanthemum morifolium  

作　　者：Lijie Zhou Shenhui Liu Yiguang Wang Yuxi Wang Aiping Song Jiafu Jiang Sumei Chen Zhiyong Guan Fadi Chen 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,The Key Laboratory of Landscaping,Ministry of Agriculture and Rural Affairs,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China

出　　处：《Horticultural Plant Journal》2024年第1期194-204,共11页园艺学报（英文版）

基　　金：financially supported grants from National Natural Science Foundation of China(Grant Nos.31902053,31870279,31730081);China Postdoctoral Science Foundation(Grant No.2018M642273);Jiangsu Planned Projects or Postdoctoral Reaearch Funds(Grant No.2019K169);the Fundamental Research Funds for the Central Uniersities(Grant No.KYQN202031);the National Key Research and Development Program of China(Grant Nos.2019YFD1001500,2020YFD1000400);the earmarked fund for Jiangsu Agricultural Industry Technology System,and a project funded by the Priority academic Program Development of Jiangsu Higher Education Institutions。

摘　　要：Color fading caused by a decrease in anthocyanin accumulation during the post-flowering stage significantly affects postharvest quality of chrysanthemum.However,the underlying mechanism by which anthocyanin accumulation decreases during the post-flowering stage still unclear,which greatly restricts design of molecular breeding in chrysanthemum.Here,a chrysanthemum SG7 R2R3 MYB transcription factor(TF),CmMYB3-like,was identified to have a function in regulating anthocyanin biosynthesis during the post-flowering stage.Quantitative real time PCR(qRT-PCR)assays showed that the expression of CmMYB3-like was gradually downregulated when anthocyanin content increased during the flowering stage and was significantly upregulated during the post-flowering stage.Genetic transformation of chrysanthemum and dual-luciferase assays in N.benthamiana leaves showed that CmMYB3-like suppressed anthocyanin accumulation by inhibiting the transcription of CmCHS and CmANS directly and that of CmF3H indirectly.However,overexpression or suppression of CmMYB3-like did not affect the biosynthesis of flavones or flavonols.Genetic transformation of chrysanthemum revealed that the overexpression of CmMYB3-like inhibited anthocyanin accumulation,but its suppression prevented the decrease in anthocyanin accumulation during the post-flowering stage.Our results revealed a crucial role of CmMYB3-like in regulating the color of petals during the post-flowering stage and provided a target gene for molecular design breeding to improve the postharvest quality of chrysanthemum.

关 键 词：Post-flowering stage Color fading Anthocyanins CmMYB3-like CHRYSANTHEMUM Molecular breeding 

分 类 号：S682.11[农业科学—观赏园艺]