GAMYB transcription factor LoMYB65 from lily plays a vital role in pollen development  

作　　者：Xinyue Liu Ling He Ze Wu Nianjun Teng 

机构地区：[1]Key Laboratory of Landscaping Agriculture,Ministry of Agriculture and Rural Affairs,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [2]Key Laboratory of Biology of Ornamental Plants in East China,National Forestry and Grassland Administration,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [3]Jiangsu Graduate Workstation of Nanjing Agricultural University and Nanjing Oriole Island Modern Agricultural Development Co.,Ltd.,Nanjing,Jiangsu 210043,China

出　　处：《Horticultural Plant Journal》2024年第1期223-238,共16页园艺学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFD1200500);the Fundamental Research Funds for the Central Universities(KYZZ2022004);the Project for Crop Germplasm Resources Conservation of Jiangsu(2021-SJ-011);the High Level Talent Project of the Top Six Talents in Jiangsu(NY-077)。

摘　　要：Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination problems.There are many studies on pollen development in model plants,but few on flower crops such as lilies.Gibberellin(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive development.GAMYB is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther development.Here,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in Arabidopsis.Fluorescence quantitative PCR results showed that LoMYB65 was mainly expressed in lily anthers.LoMYB65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf cells.The conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its transactivation.Overexpression of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male sterile.Simultaneously,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen amount.Overexpression of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be dose-dependent.Overall,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in lily.LoMYB65 may provide a useful candidate gene for pollenless breeding of lily.

关 键 词：LILY Anther development Pollen pollution GAMYB VIGS 

分 类 号：S682.29[农业科学—观赏园艺]