Styrax japonicus functional genomics:an efficient virus induced gene silencing(VIGS)system  

作　　者：Gangyu Sun Yiqian Ju Cuiping Zhang Lulu Li Xinqiang Jiang Xiaoman Xie Yizeng Lu Kuiling Wang Wei Li 

机构地区：[1]College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao,Shandong 266109,China [2]Shandong Provincial Center of Forest and Grass Germplasm Resources,Jinan,Shandong 250102,China

出　　处：《Horticultural Plant Journal》2024年第1期252-258,共7页园艺学报（英文版）

基　　金：supported by the Subject of Key R&D Plan of Shandong Province(Major Scientific and Technological Innovation Project)“Mining and Accurate Identification of Forest Tree Germplasm Resources”(Grant Nos.2021LZGC02303);Science&Technology Specific Projects in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta(Grant No.022SZX16)。

摘　　要：VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is limited.In this study,we compared the characteristics that could affect the viability of VIGS in S.japonicus,including the acetosyringone(AS)concentration,the Agrobacterium’s optical density and the inoculation method.The stable reference genes of S.japonicus were selected to validate the gene’s knockdown by quantitative PCR.As a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200μmol·L^(-1)and OD600of 0.5,and friction-osmosis with AS concentration of 200μmol·L^(-1)and OD600of 1.0,which silencing efficiency was 83.33%and 74.19%,respectively.The successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in S.japonicus to identify unknown gene functions.

关 键 词：Styrax japonicus VIGS Reference gene 

分 类 号：Q943.2[生物学—植物学]