Allotetraploidization event of Coptis chinensis shared by all Ranunculales  

作　　者：Yan Zhang Weina Ge Jia Teng Yanmei Yang Jianyu Wang Zijian Yu Jiaqi Wang Qimeng Xiao Junxin Zhao Shaoqi Shen Yishan Feng Shoutong Bao Yu Li Yuxian Li Tianyu Lei Yuxin Pan Lan Zhang Jinpeng Wang 

机构地区：[1]Department of Bioinformatics,School of Life Sciences,North China University of Science and Technology,Tangshan,Hebei 063000,China [2]School of Life Sciences,North China University of Science and Technology,Tangshan,Hebei 063000,China [3]State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Science,Beijing 100093,China [4]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Horticultural Plant Journal》2024年第1期288-303,共16页园艺学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(Grant Nos.32170236 and 31501333);the Natural Science Foundation of Hebei Province(Grant No.C2020209064);the Youth Foundation of Educational Committee of Hebei Province(Grant No.QN2020139)。

摘　　要：Coptis chinensis Franch.,also named Chinese goldthread is a member of Ranunculaceae in the order Ranunculales and represents an important lineage of early eudicots with traditional medicinal value.In our study,by using syntenic analysis combined with phylogenomic analysis of C.chinensis and four other representative genomes from basal and core eudicots,we confirmed that the WGD event in C.chinensis was shared by Aquilegia coerulea and Papaver somniferum L.and quickly occurred after Ranunculales diverged from other eudicots,likely a Ranunculales common tetraploidization(RCT).The synonymous nucleotide substitutions at synonymous sites distribution of syntenic blocks across these genomes showed that the evolutionary rate of the P.somniferum genome is faster than that of the C.chinensis genome by approximately 13.7%,possibly due to Papaveraceaes having an additional special tetraploidization event(PST).After Ks correction,the RCT dated to 115—130 million years ago(MYA),which was close to the divergence of Ranunculaceaes and Papaveraceaes approximately115.45—130.51 MYA.Moreover,we identified homologous genes related to polyploidization and speciation and constructed multiple sequence alignments with different reference genomes.Notably,the event-related subgenomes in the basal genomes all showed genomic fractionation bias,suggesting a likely allopolyploid nature of the RCT,PST and T-Alpha and T-Beta events in Tetracentron sinense.In addition,we detected that the sixteen P450 subfamilies were markedly expanded in the genomes of Ranunculales,and most of them were related to the RCT and PST events.We constructed a new platform for Early Eudicot Comparative Genomic Research(http://www.cgrpoee.top/index.html)to store more information.In summary,our findings support the WGD of C.chinensis shared by Ranunculales,which is likely an allotetraploidization event.This present effort offered new insights into the evolution of key polyploidization events and the genes related to secondary metabolites during the diversification o

关 键 词：Coptis chinensis RANUNCULALES POLYPLOIDIZATION Genomic fractionation P450 genes 

分 类 号：S567.52[农业科学—中草药栽培]