军事航空噪声引起的隐匿性听力损失小鼠存在耳蜗核神经元过度激活  

Hyperactivation of cochlear nucleus neurons in mice with military aviation noise-induced hidden hearing loss

作　　者：朱靖 姜一弘 吴侃 郑泽宇 张鹏 畅通 张卓儒 王菲 王昊天 张敏 王小成 ZHU Jing;JIANG Yihong;WU Kan;ZHENG Zeyu;ZHANG Peng;CHANG Tong;ZHANG Zhuoru;WANG Fei;WANG Haotian;ZHANG Min;WANG Xiaocheng(Center of Clinical Aerospace Medicine,School of Aerospace Medicine,Key Laboratory of Aerospace Medicine of Ministry of Education,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China;Department of Aviation Medicine,Xijing Hospital,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China;No.3 Cadet Regiment,School of Basic Medical Sciences,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China;No.4 Cadet Regiment,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学航空航天医学系航空航天临床医学中心,航空航天医学教育部重点实验室,陕西西安710032 [2]空军军医大学西京医院空勤科,陕西西安710032 [3]空军军医大学基础医学院学员三大队,陕西西安710032 [4]空军军医大学基础医学院学员四大队,陕西西安710032

出　　处：《空军军医大学学报》2024年第2期169-174,共6页Journal of Air Force Medical University

基　　金：空军装备科研重点项目(KJ2020XXXXX223);空军军医大学“珠峰工程”军事医学人才基金(4139Z4113H32);空军军医大学航空航天医学一流学科建设引导专项优博优硕支持计划项目(2022YSZC03)。

摘　　要：目的构建小鼠隐匿性听力损失(HHL)和噪声性听力损失模型,分析军事航空噪声暴露对小鼠耳蜗核神经元激活状态的影响。方法将听力正常的雄性C57小鼠随机分成3组,分别给予110 dB 2 h噪声、115 dB 4 h噪声和不给噪声刺激。在噪声暴露后1、7、14、28 d进行听性脑干反应(ABR)测试和小鼠耳蜗核c-Fos蛋白免疫荧光染色。结果HHL组小鼠噪声暴露后听阈发生暂时性阈移,阈上刺激ABRⅠ波幅值降低,耳蜗核c-Fos蛋白表达阳性神经元数量增多。结论军事航空噪声引起小鼠HHL表现为听觉暂时性阈移、阈上刺激ABRⅠ波幅值降低,并且耳蜗核神经元出现过度激活。Objective To construct mouse models of hidden hearing loss(HHL) and noise-induced hearing loss and analyze the effects of military aviation noise exposure on the activation state of cochlear nucleus neurons in mice.Methods Male C57 mice with normal hearing were randomly divided into 3 groups and given 110 dB 2 h noise,115 dB 4 h noise and no noise exposure,respectively.Auditory brainstem response(ABR) tests and immunofluorescence staining of c-Fos protein in mouse cochlear nuclei were performed at 1,7,14 and 28 d after noise exposure.Results After noise exposure,the auditory threshold of the mice in HHL group experienced a temporary threshold shift,with a decrease in ABR Ⅰ amplitude of supraliminal stimuli and an increase in the number of positive neurons expressing c-Fos protein in the cochlear nucleus.Conclusion Military aviation noise-induced HHL in mice is characterized by a temporary shift in auditory thresholds,a decrease in ABR Ⅰ wave amplitude of supraliminal stimuli,and hyperactivation of cochlear nucleus neurons.

关 键 词：隐匿性听力损失 军事航空噪声 听性脑干反应 耳蜗核 C-FOS蛋白 

分 类 号：R764[医药卫生—耳鼻咽喉科]