化湿疗法对自发型干燥综合征小鼠腺体功能和腺体病理状态的影响  

Effect of eliminating dampness therapy on gland function and gland pathological state of mice with spontaneous Sjogren's syndrome

作　　者：于溪 李成荫[2] YU Xi;LI Cheng-yin(Hunan University of Traditional Chinese Medicine,Changsha 410280,China)

机构地区：[1]湖南中医药大学,长沙410280 [2]重庆市中医院

出　　处：《山西中医》2024年第2期55-58,共4页Shanxi Journal of Traditional Chinese Medicine

摘　　要：目的:初步探索化湿疗法对干燥综合征的作用机制。方法:将自发型干燥综合征模型NOD/Ltj小鼠随机分为化湿组、养阴化湿组、模型组,选取ICR小鼠作为对照组。对照组和模型组予生理盐水灌胃,其他组予相应的药物治疗。药物治疗16周。灌胃期间检测小鼠的唾液流率,灌胃结束后处死小鼠,取颌下腺进行病理评价,采用流式细胞术检测脾脏及外周血中淋巴结髓源性抑制细胞(MDSC)的比例。结果:与对照组比较,模型组小鼠的唾液流率明显下降(P<0.05或P<0.01),外周血中的MDSC水平高于对照组(P<0.05),脾脏中的MDSC水平较对照组有升高趋势(P>0.05),颌下腺淋巴细胞浸润面积与总面积比例明显高于对照组(P<0.05)。与模型组比较,12周龄、18周龄、21周龄时化湿组和养阴化湿组小鼠唾液流率均明显升高,外周血及脾脏中的MDSC水平均显著降低,两组小鼠颌下腺淋巴细胞浸润面积与总面积比例有下降趋势,但无统计学差异(P>0.05)。结论:化湿疗法可以促进干燥综合征模型小鼠唾液腺分泌,降低外周血及脾脏中的MDSC水平,对干燥综合征有显著的治疗作用。Objective:To approach the mechanism of eliminating dampness therapy on Sjogren's syndrome.Methods:NOD/Ltj mice of spontaneous Sjogren's syndrome model were randomly divided into eliminating dampness group,nourishing yin to eliminate dampness group and model group.ICR mice were selected as control group.The control group and model group were given normal saline by gavage,while the other groups were given corresponding drugs for treatment.The treatment with drug lasted for 16 weeks.The salivary flow rate of mice was detected during the gavage.After the gavage,the mice were sacrificed,the submandibular glands were taken out and pathologically evaluated,and the proportion of marrow-derived suppressor cells(MDSC)of cervical lymph nodes in the spleen and peripheral blood was detected by flow cytometry.Results:Compared with the control group,the salivary flow rate of the mice in model group was significantly decreased(P﹤0.05 or P﹤0.01);the proportion of MDSC in peripheral blood was higher than that in the control group(P﹤0.05);the level of MDSC in the spleen showed a rising trend compared with the control group(P﹥0.05);and the level of lymphocyte infiltration area to total area in submandibular gland was significantly higher than that in the control group(P﹤0.05).Compared with the model group,the saliva flow rate of the mice in eliminate dampness group and nourishing yin to eliminate dampness group was significantly increased at 12 weeks of age,18 weeks of age and 21 weeks of age;both in eliminate dampness group and in nourishing yin to eliminate dampness group,the level of MDSC in peripheral blood and spleen was significantly reduced;and in the two groups,the proportion of lymphocyte infiltration area to total area in submandibular gland of mice showed a downward trend,but there was no significant statistical difference(P﹥0.05).Conclusion:Eliminating dampness therapy can promote the secretion of salivary glands in mice of Sjogren's syndrome model,reduce the level of MDSC in peripheral blood and spleen,and h

关 键 词：干燥综合征 化湿疗法 白术 苍术 实验研究 

分 类 号：R442.8[医药卫生—诊断学]