Telomere-to-telomere carrot (Daucus carota) genome assembly reveals carotenoid characteristics  

作　　者：Ya-Hui Wang Pei-Zhuo Liu Hui Liu Rong-Rong Zhang Yi Liang Zhi-Sheng Xu Xiao-Jie Li Qing Luo Guo-Fei Tan Guang-Long Wang Ai-Sheng Xiong 

机构地区：[1]State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [2]Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550025,China [3]School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huaian,Jiangsu 223003,China [4]Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in North China,Beijing 100097,China

出　　处：《Horticulture Research》2023年第7期112-124,共13页园艺研究（英文）

基　　金：This article was supported by National Natural Science Foundation of China(32072563,32102369);Natural Science Foundation of Jiangsu Province(BK20211366);Project of Guizhou Provincial Department of Science and Technology(Qiankehe Fuqi[2022005]);Project of Guiyang of Science and Technology Bureau(Zhuke contract[2021]5-1);Priority Academic Program Development of Jiangsu Higher Education Institutions Project(PAPD).

摘　　要：Carrot(Daucus carota)is an Apiaceae plant with multi-colored fleshy roots that provides a model system for carotenoid research.In this study,we assembled a 430.40 Mb high-quality gapless genome to the telomere-to-telomere(T2T)level of“Kurodagosun”carrot.In total,36268 genes were identified and 34961 of them were functionally annotated.The proportion of repeat sequences in the genome was 55.3%,mainly long terminal repeats.Depending on the coverage of the repeats,14 telomeres and 9 centromeric regions on the chromosomes were predicted.A phylogenetic analysis showed that carrots evolved early in the family Apiaceae.Based on the T2T genome,we reconstructed the carotenoid metabolic pathway and identified the structural genes that regulate carotenoid biosynthesis.Among the 65 genes that were screened,9 were newly identified.Additionally,some gene sequences overlapped with transposons,suggesting replication and functional differentiation of carotenoid-related genes during carrot evolution.Given that some gene copies were barely expressed during development,they might be functionally redundant.Comparison of 24 cytochrome P450 genes associated with carotenoid biosynthesis revealed the tandem or proximal duplication resulting in expansion of CYP gene family.These results provided molecular information for carrot carotenoid accumulation and contributed to a new genetic resource.

关 键 词：CARROT sequences CHARACTERISTICS 

分 类 号：S631.2[农业科学—蔬菜学]