FvWRKY50 is an important gene that regulates both vegetative growth and reproductive growth in strawberry  

作　　者：Yating Chen Liping Liu Qianqian Feng Chuang Liu Yujuan Bao Nan Zhang Ronghui Sun Zhaonan Yin Chuanfei Zhong Yuanhua Wang Qian Li Bingbing Li 

机构地区：[1]Department of Pomology,College of Horticulture,China Agricultural University,Beijing,10093,China [2]Beijing Engineering Research Center for Strawberry,Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100093,China [3]Department of Agronomy and Horticulture,Jiangsu Vocational College of Agriculture and Forestry,Jiangsu,212400,China [4]Engineering and Technical Center for Modern Horticulture,Jiangsu,212400,China

出　　处：《Horticulture Research》2023年第7期280-292,共13页园艺研究（英文）

基　　金：Thisworkwas supported by the National Natural Science Foundation of China(32222074,32072551);the National Key Research and Development Program of China(2022YFD2100102-3);111 Project(B17043);Beijing Innovation Consortium of Agriculture Research System(BAIC04-2022);the 2115 Talent Development Program of China Agricultural University.

摘　　要：The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.

关 键 词：STRAW VEGETATIVE BERRY 

分 类 号：S668.4[农业科学—果树学]