VqMAPK3/VqMAPK6, VqWRKY33, and VqNSTS3 constitute a regulatory node in enhancing resistance to powdery mildew in grapevine  

作　　者：Wandi Liu Chaohui Yan Ruimin Li Guanyu Chen Xinqi Wang Yingqiang Wen Chaohong Zhang Xiping Wang Yan Xu Yuejin Wang 

机构地区：[1]College of Horticulture,Northwest A&F University,Yangling,Shaanxi,712100,China [2]Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture,Yangling,Shaanxi,712100,China [3]State Key Laboratory of Crop Stress Biology in Arid Areas,Northwest A&F University,Yangling,Shaanxi,712100,China

出　　处：《Horticulture Research》2023年第7期293-308,共16页园艺研究（英文）

基　　金：The research was funded by the National Natural Science Foundation of China(grant no.32272667).

摘　　要：Grapevine powdery mildew is caused by Erysiphe necator,which seriously harms grape production in the world.Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew.A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2.The novel VqNSTS3 was transferred into susceptible‘Thompson Seedless’by Agrobacterium-mediated transformation.The transgenic plants showed resistance to the disease and activated other resistance-related genes.VqNSTS3 expression in grapevine is regulated by VqWRKY33,and which binds to TTGACC in the VqNSTS3 promoter.Furthermore,VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased VqNSTS3 expression.ProVqNSTS3::VqNSTS3-GFP of transgenic VqNSTS3 in Arabidopsis thaliana was observed to move to and wrap the pathogen’s haustoria and block invasion by Golovinomyces cichoracearum.These results demonstrate that stilbene accumulation of novel VqNSTS3 of the Chinese wild Vitis quinquangularis accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew.Therefore,VqNSTS3 can be used in generating powdery mildew-resistant grapevines.

关 键 词：INVASION expression. RESISTANCE 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治]