Multiple-statistical genome-wide association analysis and genomic prediction of fruit aroma and agronomic traits in peaches  

作　　者：Xiongwei Li Jiabo Wang Mingshen Su Minghao Zhang Yang Hu Jihong Du Huijuan Zhou Xiaofeng Yang Xianan Zhang Huijuan Jia Zhongshan Gao Zhengwen Ye 

机构地区：[1]Peach Research Department of Forest&Fruit Tree Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China [2]Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization(Southwest Minzu University,Ministry of Education),Chengdu,Sichuan 610041,China [3]Peach Group of Shanghai Runzhuang Agricultural Science and Technology Institute,Shanghai 201415,China [4]Department of Horticulture,Key Laboratory for Horticultural Plant Growth,Development and Quality Improvement of State Agriculture Ministry,Zhejiang Unihversity,Hangzhou 310058,China

出　　处：《Horticulture Research》2023年第7期309-320,共12页园艺研究（英文）

基　　金：This work was supported by funds from the National Key Research and Development Program of China(2019YFD1000801);the Science and Technology Commission of Shanghai Municipality(23 N11900400);Hu Nong Ke Chuang Zi(2021)1-1,Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties,China(2021C02066-4);the Outstanding Team Program of Shanghai Academy of Agricultural Science(2022-004);the‘Pangao’Program of Shanghai Academy of Agricultural Science.

摘　　要：‘Chinese Cling’is an important founder in peach breeding history due to the pleasant flavor.Genome-wide association studies(GWAS)combined with genomic selection are promising tools in fruit tree breeding,as there is a considerable time lapse between crossing and release of a cultivar.In this study,242 peaches from Shanghai germplasm were genotyped with 145456 single-nucleotide polymorphisms(SNPs).The six agronomic traits of fruit flesh color,fruit shape,fruit hairiness,flower type,pollen sterility,and soluble solids content,along with 14 key volatile odor compounds(VOCs),were recorded for multiple-statistical GWAS.Except the reported candidate genes,six novel genes were identified as associated with these traits.Thirty-nine significant SNPs were associated with eight VOCs.The putative candidate genes were confirmed for VOCs by RNA-seq,including three genes in the biosynthesis pathway found to be associated with linalool,soluble solids content,and cis-3-hexenyl acetate.Multiple-trait genomic prediction enhanced the predictive ability forγ-decalactone to 0.7415 compared with the single-trait model value of 0.1017.One PTS1-SSR marker was designed to predict the linalool content,and the favorable genotype 187/187 was confirmed,mainly existing in the‘Shanghai Shuimi’landrace.Overall,our findings will be helpful in determining peach accessions with the ideal phenotype and show the potential of multiple-trait genomic prediction to improve accuracy for highly correlated genetic traits.The diagnostic marker will be valuable for the breeder to bridge the gap between quantitative trait loci and marker-assisted selection for developing strong-aroma cultivars.

关 键 词：breeding CULTIVAR FIR 

分 类 号：S662.1[农业科学—果树学]