Chromosome-scale genome assembly of Prunus pusilliflora provides novel insights into genome evolution, disease resistance, and dormancy release in Cerasus L.  

作　　者：Songtao Jiu Baozheng Chen Xiao Dong Zhengxin Lv Yuxuan Wang Chunjin Yin Yan Xu Sen Zhang Jijun Zhu Jiyuan Wang Xunju Liu Wanxia Sun Guoqian Yang Meng Li Shufeng Li Zhuo Zhang Ruie Liu Lei Wang Muhammad Aamir Manzoor Quero-García José Shiping Wang Yahui Lei Ling Yang Elisabeth Dirlewanger Yang Dong Caixi Zhang 

机构地区：[1]Department of Plant Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai,200240,P.R.China [2]College of Food Science and Technology,Yunnan Agricultural University,Kunming,Yunnan Province,650201,P.R.China [3]Dali Bai Autonomous Prefecture Academy of Agricultural Sciences and Extension,Dali,Yunnan Province,671600,P.R.China [4]Shanghai Botanical Garden,Shanghai,200231,P.R.China [5]College of Biology and the Environment,Nanjing Forestry University,Nanjing,Jiangsu Province,200037,P.R.China [6]INRAe,UMR 1332 de Biologie du Fruit et Pathologie,33140 Villenave d’Ornon,France

出　　处：《Horticulture Research》2023年第5期220-235,共16页园艺研究（英文）

基　　金：This study was funded by the Shanghai Agriculture Applied Technology Development Program,China(grant no.2022-02-08-00-12-F01111);the China Agriculture Research System(grant no.CARS-30-2-08);the National Natural Science Foundation of China(grant no.32102347);the Shanghai Sailing Program(grant no.21YF1422100);the Startup Fund for Young Faculty at SJTU(grant no.21X010500643).

摘　　要：Prunus pusilliflora is a wild cherry germplasm resource distributed mainly in Southwest China.Despite its ornamental and economic value,a high-quality assembled P.pusilliflora genome is unavailable,hindering our understanding of its genetic background,population diversity,and evolutionary processes.Here,we de novo assembled a chromosome-scale P.pusilliflora genome using Oxford Nanopore,Illumina,and chromosome conformation capture sequencing.The assembled genome size was 309.62 Mb,with 76 scaffolds anchored to eight pseudochromosomes.We predicted 33035 protein-coding genes,functionally annotated 98.27%of them,and identified repetitive sequences covering 49.08%of the genome.We found that P.pusilliflora is closely related to Prunus serrulata and Prunus yedoensis,having diverged from them∼41.8 million years ago.A comparative genomic analysis revealed that P.pusilliflora has 643 expanded and 1128 contracted gene families.Furthermore,we found that P.pusilliflora is more resistant to Colletotrichum viniferum,Phytophthora capsici,and Pseudomonas syringae pv.tomato(Pst)DC3000 infections than cultivated Prunus avium.P.pusilliflora also has considerably more nucleotide-binding site-type resistance gene analogs than P.avium,which explains its stronger disease resistance.The cytochrome P450 and WRKY families of 263 and 61 proteins were divided into 42 and 8 subfamilies respectively in P.pusilliflora.Furthermore,81 MADS-box genes were identified in P.pusilliflora,accompanying expansions of the SVP and AGL15 subfamilies and loss of the TM3 subfamily.Our assembly of a high-quality P.pusilliflora genome will be valuable for further research on cherries and molecular breeding.

关 键 词：PRUNUS resistance RELEASE 

分 类 号：S662.3[农业科学—果树学]