Nicotiana benthamiana XYLEM CYSTEINE PROTEASE genes facilitate tracheary element formation in interfamily grafting  

作　　者：Chaokun Huang Ken-ichi Kurotani Ryo Tabata Nobutaka Mitsuda Ryohei Sugita Keitaro Tanoi Michitaka Notaguchi 

机构地区：[1]Graduate School of Bioagricultural Sciences,Nagoya University,Furo-cho,Chikusa-ku,Nagoya 464-8601,Japan [2]Bioscience and Biotechnology Center,Nagoya University,Furo-cho,Chikusa-ku,Nagoya 464-8601,Japan [3]Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology(AIST),Tsukuba,Ibaraki 305-8566,Japan [4]Isotope Facility for Agricultural Education and Research,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Yayoi,Bunkyo-ku,Tokyo 113-8657,Japan [5]Radioisotope Research Center,Nagoya University,Furo-cho,Chikusa-ku,Nagoya 464-8602,Japan [6]Institute of Transformative Bio-Molecules,Nagoya University,Furo-cho,Chikusa-ku,Nagoya 464-8601,Japan

出　　处：《Horticulture Research》2023年第6期70-81,共12页园艺研究（英文）

基　　金：This work was supported by grants from the Japan Society for the Promotion of Science Grantsin-Aid for Scientific Research(20H03273,21H00368 and 21H05657 toMNand 22 K06181 to KK);Japan Science and Technology Agency(JPMJTR194G to MN);China Scholarship Council(CSC;No.201908050204 to CH).

摘　　要：Grafting is a plant propagation technique widely used in agriculture.A recent discovery of the capability of interfamily grafting in Nicotiana has expanded the potential combinations of grafting.In this study,we showed that xylem connection is essential for the achievement of interfamily grafting and investigated the molecular basis of xylem formation at the graft junction.Transcriptome and gene network analyses revealed gene modules for tracheary element(TE)formation during grafting that include genes associated with xylem cell differentiation and immune response.The reliability of the drawn network was validated by examining the role of the Nicotiana benthamiana XYLEM CYSTEINE PROTEASE(NbXCP)genes in TE formation during interfamily grafting.Promoter activities of NbXCP1 and NbXCP2 genes were found in differentiating TE cells in the stem and callus tissues at the graft junction.Analysis of a Nbxcp1;Nbxcp2 loss-of-function mutant indicated that NbXCPs control the timing of de novo TE formation at the graft junction.Moreover,grafts of the NbXCP1 overexpressor increased the scion growth rate as well as the fruit size.Thus,we identified gene modules for TE formation at the graft boundary and demonstrated potential ways to enhance Nicotiana interfamily grafting.

关 键 词：GRAFTING ELEMENT GRAFT 

分 类 号：S339.45[农业科学—作物遗传育种] S572[农业科学—农艺学]