Genomic features of meiotic crossovers in diploid potato  

作　　者：Xiuhan Jiang Dawei Li Hui Du Pei Wang Liang Guo Guangtao Zhu Chunzhi Zhang 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China [2]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China [3]The AGISCAAS-YNNU Joint Academy of Potato Sciences,Yunnan Normal University,Kunming,Yunnan 650500,China

出　　处：《Horticulture Research》2023年第6期144-153,共10页园艺研究（英文）

基　　金：This work was supported by the China National Key Research and Development Program(Grant Number 2019YFE0120500);the National Natural Science Foundation of China(32022075);the Natural Science Foundation of Shenzhen(JCYJ20190813142201666);the National Science Fund of Yunnan for Distinguished Young Scholars(Grant No.202001AV070003);the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202101);Science Technology and Innovation Commission of Shenzhen Municipality of China(ZDSYS20200811142605017).

摘　　要：Meiotic recombination plays an important role in genome evolution and crop improvement.Potato(Solanum tuberosum L.)is the most important tuber crop in the world,but research about meiotic recombination in potato is limited.Here,we resequenced 2163 F2 clones derived from five different genetic backgrounds and identified 41945 meiotic crossovers.Some recombination suppression in euchromatin regions was associated with large structural variants.We also detected five shared crossover hotspots.The number of crossovers in each F2 individual from the accession Upotato 1 varied from 9 to 27,with an average of 15.5,78.25%of which were mapped within 5 kb of their presumed location.We show that 57.1%of the crossovers occurred in gene regions,with poly-A/T,poly-AG,AT-rich,and CCN repeats enriched in the crossover intervals.The recombination rate is positively related with gene density,SNP density,Class II transposon,and negatively related with GC density,repeat sequence density and Class I transposon.This study deepens our understanding of meiotic crossovers in potato and provides useful information for diploid potato breeding.

关 键 词：BREEDING TUBEROSUM CROSSOVER 

分 类 号：S532[农业科学—作物学] S33