玉米花丝响应干旱胁迫的转录组学分析  

Transcriptomic Analysis of Maize Silks in Response to Drought Stress

作　　者：朱凡 徐芷琪 段雨洁 李阳阳 宋有洪 ZHU Fan;XU Zhiqi;DUAN Yujie;LI Yangyang;SONG Youhong(College of Agronomy,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学农学院,安徽合肥230036

出　　处：《华北农学报》2024年第1期37-47,共11页Acta Agriculturae Boreali-Sinica

基　　金：安徽省自然科学基金项目(2208085MC59)。

摘　　要：为探究花丝对干旱胁迫的分子响应,以安农591和先玉335为材料,设置土壤含水量为80%田间持水量(CK)和60%田间持水量(DS)的2个处理,在玉米花丝伸长速增期取样,进行转录组测序。通过对比2个品种干旱组和对照组的基因表达差异,明确玉米花丝响应干旱的关键通路和相关候选基因。结果表明:苯丙烷类生物合成途径(ko00940)和淀粉和蔗糖代谢途径(ko00500)为玉米花丝响应干旱的关键通路。在苯丙烷类生物合成途径通路中编码4-香豆酸-辅酶A连接酶(4CL)、肉桂酰辅酶A还原酶(CCR)、肉桂醇脱氢酶(CAD)和过氧化物酶(POD)的基因在干旱的玉米花丝中低表达。相关分析表明,编码4CL、CCR、CAD和POD的基因下调与木质素含量降低有关。在淀粉和蔗糖代谢途径中转化酶和蔗糖合酶基因在干旱的玉米花丝中低表达;相关分析表明,转化酶和蔗糖合酶基因下调与蔗糖代谢有关。综上所述,本研究确定了参与蔗糖和木质素代谢的关键基因和代谢物,木质素合成可能与玉米花丝耐旱相关,4CL、CCR、CAD和POD是木质素合成通路中玉米花丝耐旱的候选基因;此外,淀粉和蔗糖代谢途径可能与花丝伸长和花丝耐旱有关,转化酶和蔗糖合酶可能是调控花丝耐旱的关键酶。To investigate the molecular response to drought stress of silks,two treatments with soil moisture content of 80%field holding capacity(CK)and 60%field holding capacity(DS)were set up using Annong 591 and Xianyu 335,and the maize silks were sampled during the rapid increase in silk elongation,and transcriptome sequencing was performed.By comparing the gene expression differences between the drought and control groups of the two varieties,the key pathways and related candidate genes of maize silks in response to drought were clarified.The results showed that the phenylpropane biosynthesis pathway(ko00940)and starch and sucrose metabolism pathway(ko00500)were the key pathways in maize silk response to drought.In the phenylpropane biosynthetic pathway 4CL,CCR,CAD and POD genes were lowly expressed in drought-prone maize silks.Correlation analysis showed that down-regulation of 4CL,CCR,CAD and POD genes was associated with reduced lignin content.Converting enzyme and sucrose synthase genes in the starch and sucrose metabolic pathways were lowly expressed in drought maize silks.Correlation analysis indicated that down-regulation of convertase and sucrose synthase genes was associated with sucrose metabolism.In summary,this study identified key genes and metabolites involved in sucrose and lignin metabolism.The lignin synthesis are associated with drought tolerance in maize silks,and 4CL,CCR,CAD,and POD are the candidate genes for drought tolerance in maize silks.In addition,the starch and sucrose metabolic pathways are associated with silk elongation and drought tolerance of silks,and invertase and sucrose synthase are the main candidate key enzymes.

关 键 词：玉米 花丝 干旱胁迫 转录组 苯丙烷类生物合成途径 淀粉和蔗糖代谢途径 

分 类 号：S513.03[农业科学—作物学] Q78[生物学—分子生物学]