基于全基因组预测和分析花生果腐病原菌分泌蛋白  

Whole Genome-Based Prediction and Analysis of Secreted Proteins of Peanut Fruit Rot Pathogens

作　　者：王辉灵 韩会玲 王晓晗 焦镇 李步阳 何弯弯 孙伟明 WANG Huiling;HAN Huiling;WANG Xiaohan;JIAO Zhen;LI Buyang;HE Wanwan;SUN Weiming(Hebei Normal University of Science and Technology,College of Marine Resources and Environment/College of Agriculture and Biotechnology/Key Laboratory of Crop Stress Biology in Hebei Province(Under Preparation),Qinhuangdao 066000,Hebei,China;Hebei Peanut Fertilizer Research Institute Co.,Ltd,Hengshui 053000,Hebei,China)

机构地区：[1]河北科技师范学院海洋资源与环境学院/农学与生物科技学院/河北省作物逆境生物学重点实验室(筹),河北秦皇岛066000 [2]河北长生果肥料研究院有限公司,河北衡水053000

出　　处：《四川农业大学学报》2024年第1期11-18,38,共9页Journal of Sichuan Agricultural University

基　　金：河北省重点研发计划项目(花生现代种业科技创新团队21326316D-6);河北省现代农业产业技术体系油料创新团队(HBCT2023030206);河北省自然科学基金青年科学基金项目(C2021407021)。

摘　　要：【目的】为分析花生果腐病病原菌-新孢镰刀菌的侵染机制并筛选可用基因。【方法】以全基因组测序结果为基础,根据分泌蛋白与效应因子的特点,采用SignalP、TMHMM、WoLF PSORT及NetGPI等软件一步步筛选,对新孢镰刀菌全基因组12756条蛋白序列进行了分泌蛋白预测和功能分析。【结果】新孢镰刀菌全基因组编码蛋白中有701条序列具有典型的分泌蛋白特征,占蛋白总数的5.49%,其长度主要分布在101~700个氨基酸范围内。信号肽的分析结果表明,信号肽-3和-1位置的氨基酸相对保守,其切割位点属于典型的A-X-A型。对分泌蛋白功能预测结果表明,656个分泌蛋白获得了功能注释,其功能主要涉及碳水化合物的运输、代谢过程,蛋白翻译后修饰和氨基酸代谢、运输过程。分泌蛋白中效应蛋白预测结果表明,新孢镰刀菌分泌蛋白中共有161个潜在的效应蛋白。【结论】对新孢镰刀菌分泌蛋白的有效预测和功能分析,为筛选新效应蛋白,明确其致病机理,筛选寄主抗性基因奠定了基础。同时为花生果腐病抗性品种选育和综合防治提供理论依据。【Objective】This study is to analyze the infection mechanism of Fusarium neocosmosporiel⁃lum,the pathogen of peanut pod rot and to select candidate genes.【Method】Based on the data of whole genome sequencing,the character of secreted proteins and effector proteins,prediction of the secreted protein and functional analysis for all the 12756 protein sequences of the whole genome of F.neocosmos⁃poriellum were carried out using software of SignalP,TMHMM,WoLF PSORT,and NetGPI.【Result】The results showed that 701 sequences encoded proteins in the whole genome of F.neocosmosporiellum had typical secretory protein characteristics,accounting for 5.49%of the total proteins,and their lengths ranged from 101 to 700 amino acids.The analysis results of the signal peptide showed that the amino acids at site-3 and site-1 of the signal peptide were relatively conserved,and the signal peptide cleavage site is of the typical A-X-A type.The function analysis showed that 656 secreted proteins were annotated,and they are mainly involved in carbohydrate transport,metabolism,protein post-translational modification,and amino acid metabolism and transport.The prediction of effector proteins showed that there are a total of 161 potential effector proteins in the secreted proteins of F.neocosmosporiellum.【Conclusion】The effective prediction and functional analysis of the secreted proteins of F.neocosmosporiel⁃lum laid the foundation for screening new effector proteins,clarifying their pathogenic mechanisms,and screening host resistance genes.Additionally,it provides a theoretical basis for breeding of peanut pod rot resistant varieties and comprehensive control of this important disease.

关 键 词：花生果腐病 新孢镰刀菌 生物信息学 分泌蛋白 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]