一株新发现虾源高致病性拟态弧菌全基因组特性的比较分析  

Comparative Analysis on the Whole Genome Characteristics of A Newly Discovered High Pathogenic Vibrio mimicus Strain Derived from Shrimp

作　　者：杨倩 贺扬 王均 古从伟 吕沐瀚 余泽辉 YANG Qian;HE Yang;WANG Jun;GU Congwei;LYU Muhan;YU Zehui(Luzhou Key Laboratory of Model Animal and Human Disease Research,Southwest Medical University,Luzhou 646000,Sichuan,China;Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,Neijiang Normal University,Neijiang 641000,Sichuan,China;The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan,China;School of Basic Medical Sciences,Zhejiang University,Hangzhou 310012,China)

机构地区：[1]西南医科大学模式动物与人类疾病研究泸州市重点实验室,四川泸州646000 [2]内江师范学院长江上游鱼类资源保护与利用四川省重点实验室,四川内江641000 [3]西南医科大学附属医院,四川泸州646000 [4]浙江大学基础医学院,杭州310012

出　　处：《四川农业大学学报》2024年第1期149-158,共10页Journal of Sichuan Agricultural University

基　　金：泸州市人民政府-西南医科大学科技战略合作项目(2020LZXNYDJ45);四川省科技厅国际合作项目(2023YFH0046)。

摘　　要：【目的】对引起日本沼虾“红体病”的拟态弧菌(Vibrio mimicus,V.mimicus)Y4菌株进行全基因组特性分析,为了解该病发病机理和控制此类疾病暴发提供参考。【方法】对Y4菌株和其他6株不同来源的拟态弧菌分离株进行了初步的比较基因组学研究。【结果】7株拟态弧菌菌株的耐药基因和tRNA数量无明显区别。Y4菌株无论是基因组大小、编码基因数量、重复序列,还是插入序列,与其他拟态弧菌菌株相比都表现出一定差异,其中IS3家族的插入序列明显多于其他拟态弧菌菌株。Y4菌株含有11个特有的毒力基因,且与多种毒力因子相关。Y4菌株基因组上还存在CRISPR序列,表明Y4菌株已获得了抵抗外来病毒基因的免疫功能。【结论】从比较基因组学的角度探究了不同来源拟态弧菌菌株的差异性,为后续拟态弧菌的生物学功能研究和基因组学研究提供了一定参考。【Objective】The genome-wide characteristic analysis on Vibrio mimicus Y4 strain which causes red body disease in Japanese swamp shrimp were performed,to provide a reference for understanding the pathogenesis of the disease and controlling of outbreaks of such diseaseas.【Method】This study conducted preliminary comparative genomics studies on the Y4 strain and six other V.mimicus isolated from the different sources.【Result】The results showed that there was no obvious difference in the antibiotic resistance genes and tRNA quantity among the seven V.mimicus strains.The Y4 strain showed certain differences compared to other V.mimicus strains,in terms of genome size,number of coding genes,repeat sequence,and insertion sequence with significantly more insertion sequences in the IS3 family than other V.mimicus strains.The Y4 strain contained 11 unique virulence genes and related to a variety of virulence factors.The genome of the Y4 strain also contained the CRISPR sequence,indicating that the Y4 strain has acquired immune function against foreign virus genes.【Conclusion】This study explored the differences in V.mimicus strains from different sources by comparative genomics analysis,which provides some references for subsequent research on the biological functions and genomics of V.mimicus.

关 键 词：虾 拟态弧菌 Y4菌株 比较基因组学 

分 类 号：S941.42[农业科学—水产养殖]