HPLC法测定蒙药甘露解毒丸中连翘酯苷A的含量研究  

Research of Content Determination for Forsythoside A in Mongolian Medicine by HPLC

作　　者：樛跃 JIU Yue(Hulunbuir Institute for Food and Drug Control,Inner Mongolia Autonomous Region,Hulunbuir 021000,China)

机构地区：[1]内蒙古自治区呼伦贝尔市食品药品检验所,内蒙古呼伦贝尔021000

出　　处：《中国民族民间医药》2024年第4期24-27,35,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立高效液相色谱法测定蒙药甘露解毒丸中连翘酯苷A的含量。方法:色谱柱为SHISEIDO CAPCELL PAK C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.4%冰醋酸(15∶85,V/V)为流动相,流速为0.8 mL/min,检测波长330nm,柱温为30℃,进样量为10μL。结果:连翘酯苷A在0.1~1.4μg范围内呈现良好线性关系,线性方程为Y=2302379X+2377(r=0.9999,n=6);专属性良好;精密度和稳定性的RSD分别为0.40%和0.80%。平均加样回收率为94.34%,RSD为1.26%(n=9)。测定两批甘露解毒丸样品,平均含量为0.7772 mg/g,RSD为0.71%。结论:高效液相色谱法测定连翘酯苷A的含量操作简便,重现性好,灵敏度较高,适用于甘露解毒丸中连翘酯苷A的含量测定。Objective To establish the high performance liquid chromatograghy method for the content determination of Forsythoside A in Mongolian medicine Ganlu Jiedu Pills.Methods The chromatographic column was SHISEIDO CAPCELL PAK C_(18)(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.4%acetic acid(15∶85,V/V),the flow rate was 0.8 mL/min,detection wavelength was 330nm,the column temperature was 30℃,and the injection volume was 10μL.Results Forsythoside A showed a good linearity in the range of 0.1-1.4μg and the linear equation was Y=2302379X+2377(r=0.9999,n=6).The specificity was good and the RSD of precision and stability were 0.40%and 0.80%.The average recovery was 94.34%with RSD of 1.26%(n=9).The average content of 2 batches samples was 0.7772 mg/g,with RSD of 0.71%.Conclusion The high performance liquid chromatograghy method for the content determination of Forsythoside A is easy to operate,reproducible and high sensitivity which can be used for the content determination of Ganlu Jiedu Pills.

关 键 词：甘露解毒丸 连翘酯苷A 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]