系统性红斑狼疮病人外周血髓样树突状细胞TLR2和TLR4表达情况及其临床意义研究  

The expression and clinical significance of TLR2 and TLR4 in peripheral blood myeloid dendritic cells of patients with systemic lupus erythematosus

作　　者：狄宁宁 方茹梦 郑倩倩 魏小松 谢长好 李志军 DI Ningning;FANG Rumeng;ZHENG Qianqian;WEI Xiaosong;XIE Changhao;LI Zhijun(Department of Rheumatology,The First Affiliated Hospital of Bengbu Medical University,Bengbu Anhui 233004;Anhui Province Key Laboratory of Immunology in Chronic Diseases,Bengbu Medical University,Bengbu Anhui 233004,China)

机构地区：[1]蚌埠医科大学第一附属医院风湿免疫科,安徽蚌埠233004 [2]慢性疾病免疫学基础与临床安徽省重点实验室,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2024年第1期40-45,共6页Journal of Bengbu Medical College

基　　金：安徽省自然科学基金项目(2108085MH258)。

摘　　要：目的:检测系统性红斑狼疮(SLE)病人外周血中髓样树突状细胞(mDC)表面Toll样受体2(TLR2)、Toll样受体4(TLR4)的表达情况及血清白细胞介素(IL)-10、IL-17A表达水平,探讨其在SLE发病机制中的作用及临床意义。方法:纳入SLE病人54例作为研究对象,根据SLEDAI-2000将SLE病人分为稳定组和活动组,同时选取本院30例健康志愿者作为对照组,应用流式细胞术检测SLE病人及健康志愿者外周血中mDC表面TLR2、TLR4的表达情况,并用ELISA法检测SLE病人及健康志愿者血清中IL-10、IL-17A的表达水平。结果:活动组SLE病人mDC-TLR2表达率高于稳定组、对照组(P<0.01),mDC-TLR4表达率高于对照组(P<0.01)。SLE病人血清中IL-10、IL-17A表达水平均高于对照组(P<0.01)。mDC-TLR2表达与抗Sm抗体呈正相关(P<0.05),与血清IgG、24 h尿蛋白定量呈负相关(P<0.05);mDC-TLR4表达与血小板计数呈正相关(P<0.05)。结论:SLE活动组病人mDC-TLR2、TLR4表达率增高,二者可能在SLE的发病或病情进展中起到一定作用。Objective:To detect the expression of Toll-like receptor 2(TLR2)and Toll-like receptor 4(TLR4)on the surface of myeloid dendritic cells(mDC)and the expression levels of serum IL-10 and IL-17A in the peripheral blood of patients with systemic lupus erythematosus(SLE),and to investigate their role in the pathogenesis of SLE and their clinical significance.Methods:A total of 54 patients with SLE were included as study subjects,and divided into stable and active groups according to the SLEDAI-2000,while 30 healthy volunteers were selected as the control group.Flow cytometry was applied to detect the expression of TLR2 and TLR4 on the surface of mDC in the peripheral blood of SLE patients and healthy volunteers,and ELISA was used to detect the expression of IL-10 and IL-17A in the serum of SLE patients and healthy volunteers.Results:The expression rate of mDC-TLR2 in SLE patients in the active group was higher than that in the stable group and control group(P<0.01).The expression rate of mDC-TLR4 was higher in the active group than that in the control group(P<0.01).The expression levels of IL-10 and IL-17A in the serum of SLE patients were higher than those in the control group(P<0.01).mDC-TLR2 expression was positively correlated with anti-Sm antibody(P<0.05),and negatively correlated with serum IgG and 24h urine protein quantification(P<0.05).The expression of mDC-TLR4 was positively correlated with platelet count(P<0.05).Conclusions:The expression rates of mDC-TLR2 and TLR4 are increased in the active SLE group,and both may play a role in the pathogenesis or progression of SLE.

关 键 词：系统性红斑狼疮 TOLL样受体 白细胞介素17A 白细胞介素10 

分 类 号：R593.241[医药卫生—内科学]