机械通气肺损伤患者微小RNA-93表达情况及临床意义  

Expression of microRNA-93 in patients with mechanical ventilation induced lung injury and its clinical significance

作　　者：刘欣[1] 林洁[1] 岳霖琳 赖纪英 李祚涛[1] LIU Xin;LIN Jie;YUE Linlin;LAI Jiying;LI Zuotao(Department of Critical Care Medicine,the First Affiliated Hospital of Gannan Medical College,Jiangxi Province,Ganzhou341000,China)

机构地区：[1]赣南医学院第一附属医院重症医学科,江西赣州341000

出　　处：《中国当代医药》2024年第5期4-8,共5页China Modern Medicine

基　　金：江西省教育厅科学技术研究项目(GJJ201511)。

摘　　要：目的探讨微小RNA-93(miR-93)在机械通气肺损伤中的表达及临床意义。方法选取2021年3月至2022年10月在赣南医学院第一附属医院ICU治疗的机械通气肺损伤患者50例作为观察组,同时选取机械通气无肺损伤患者100例作为对照组,检测两组血清miR-93、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-8(IL-8)的差异。结果观察组血清miR-93相对表达量低于对照组,差异有统计学意义(P<0.05),而TNF-α、IL-1β和IL-8高于对照组,差异有统计学意义(P<0.05)。观察组重度肺损伤患者血清miR-93相对表达量低于轻中度患者,差异有统计学意义(P<0.05),而TNF-α、IL-1β和IL-8高于轻中度患者,差异有统计学意义(P<0.05)。血清miR-93与Murray肺损伤评分呈负相关(r=-0.445,P<0.05)。血清miR-93与TNF-α、IL-1β、IL-8呈负相关(r=-0.394、-0.405、-4.111,P<0.05)。miR-93相对表达量诊断肺损伤的AUC为0.815,截断值为2.50,灵敏性和特异性分别为92.00%和58.00%;miR-93相对表达量诊断重度肺损伤的AUC为0.823,截断值为1.72,灵敏性和特异性分别为66.70%和84.40%。结论机械通气肺损伤患者血清miR-93表达下调,与患者肺损伤严重程度呈负相关,在诊断肺损伤及严重程度方面有一定应用价值。Objective To investigate the expression and clinical significance of microRNA-93(miR-93)in mechanical ventilation induced lung injury.Methods Fifty patients with mechanical ventilation lung injury treated in ICU of the First Affiliated Hospital of Gannan Medical College from March 2021 to October 2022 were selected as the observation group,and 100 patients without mechanical ventilation lung injury were selected as the control group,the serum miR-93,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-8(IL-8)were detected in the two groups.Results The relative expression of miR-93 in serum of the observation group was lower than that of the control group,the difference was statistically significant(P<0.05),while the levels of TNF-α,IL-1β and IL-8 were higher than that of the control group,the differences were statistically significant(P<0.05).The relative expression of serum miR-93 in patients with severe lung injury in the observation group was lower than that in patients with mild to moderate lung injury,the difference was statistically significant(P<0.05),while TNF-α,IL-1β and IL-8 were higher than those in patients with mild to moderate lung injury,the differences were statistically significant(P<0.05).Serum miR-93 was negatively correlated with Murray's lung injury score(r=-0.445,P<0.05).Serum miR-93 was negatively correlated with TNF-α,IL-1β and IL-8(r=-0.394,-0.405,-4.111,P<0.05).The AUC for diagnosing lung injury with the relative expression of miR-93 was 0.815,the cut-off value was 2.50,and the sensitivity and specificity were 92.00% and 58.00%,respectively.The AUC for diagnosing severe lung injury with the relative expression of miR-93 was 0.823,the cut-off value was 1.72,and the sensitivity and specificity were 66.70% and 84.40%,respectively.Conclusion The expression of serum miR-93 is down regulated in patients with mechanical ventilation lung injury,which is negatively correlated with the severity of lung injury,has certain application value in diagnosing lung injury and its

关 键 词：微小RNA-93 机械通气肺损伤 严重程度 临床意义 

分 类 号：R55[医药卫生—血液循环系统疾病]