女贞子中主要活性成分红景天苷和特女贞苷的逆转铁过载促进骨形成相互作用研究  

The reversal effect of salidroside and specnuezhenide obtained from ligustri lcidi fructus on iron overload and promotion of bone formation

作　　者：何丹丹[1,2] 赵白秀 汪华君 蒋俊[2] 沈袁佳怡[2] 何津津 HE Dandan;ZHAO Baixiu;WANG Huajun;JIANG Jun;SHEN Yuanjiayi;HE Jinjin(The Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028;School of Pharmacy,Jiangsu University,Zhenjiang 212013,Jiangsu;The Affiliated Hospital of Jiangsu University,Zhenjiang 212000,Jiangsu,China)

机构地区：[1]南京中医药大学附属中西医结合医院,江苏南京210028 [2]江苏大学药学院,江苏镇江212013 [3]江苏大学附属医院,江苏镇江212000

出　　处：《中国骨质疏松杂志》2024年第1期70-74,92,共6页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81703773);江苏省自然科学基金(BK20170560);江苏省中药骨干人才高级研修项目(SZYGG202217-1);镇江市重点研发计划-社发项目(SH2022075)。

摘　　要：目的评价女贞子中主要活性成分红景天苷和特女贞苷的逆转铁过载促进骨形成抗绝经后骨质疏松(postmenopausal osteoporosis,PMOP)效应,并探讨其相互作用关系。方法建立柠檬酸铁铵诱导的斑马鱼骨质疏松模型,分别评估红景天苷(1、5、25、50、100μmol/L)、特女贞苷(1、5、25、50、100μmol/L)及其两种成分混合溶液(1∶1)对骨矿化的影响;茜素红染色后以骨骼染色面积和累积光密度评价其骨矿化程度,并运用等效线法和等辐射分析法评价红景天苷、特女贞苷抗PMOP的相互作用。结果红景天苷、特女贞苷及其混合溶液均有较好地逆转铁过载所致的骨形成抑制作用,且在1~100μmol/L内呈现显著的浓度依赖性;在等效线法中,经半数有效浓度(EC50)计算得混合组γ>1,表明二者无明显的协同作用;在等辐射分析法中,混合组中相应成分的EC50比值分数总和为1.16,其复合点落在95%可信限内(P>0.05),表明两者呈相加作用关系,且未见明显协同作用。结论红景天苷和特女贞苷均可逆转铁过载诱导的骨形成抑制,且当红景天苷和特女贞苷联合使用时,两者呈相加作用。Objective To evaluate the effects of salidroside and specnuezhenide on reversing iron overload,promoting bone formation,and anti postmenopausal osteoporosis(PMOP),and to explore the interaction between them.Methods Zebrafish osteoporosis model induced by ferric ammonium citrate was established to evaluate the effects of salidroside(1,5,25,50,100μmol/L),specnuezhenide(1,5,25,50,100μmol/L)and their mixed solutions(1∶1)on bone mineralization.After Alizarin red staining,bone mineralization was evaluated with bone staining area and cumulative optical density.The interaction between salidroside and specnuezhenide was evaluated with Isobole method and Isobolographic analysis method.Results Salidroside,specnuezhenide,and their mixed solution reversed the inhibition of bone formation caused by iron overload,and showed a significant concentration dependence in the range of 1-100μM.The half effective concentration(EC 50)γ>1 calculated by with the Isobole method,indicating that there was no obvious synergy.The sum of the EC 50 ratio scores of the corresponding components in the mixed group was 1.16 according to the isobolographic analysis method,and its composite point fell within the 95%confidence limit(P>0.05),indicating that there was an additive relationship and there was no obvious synergistic effect.Conclusion Salidroside and specnuezhenide reverse the inhibition of bone formation caused by iron overload.The combination show additive effect.

关 键 词：女贞子 红景天苷 特女贞苷 绝经后骨质疏松 相互作用 

分 类 号：R285[医药卫生—中药学] R589[医药卫生—中医学]