EB病毒IgM、IgG及DNA检测在儿童呼吸道感染性疾病中的诊断研究  

Diagnosis of EB virus IgM,IgG and DNA in children with respiratory infectious diseases

作　　者：张悦 季忠庶 申爽[1] ZHANG Yue;JI Zhong-shu;SHEN Shuang(Huludao Central Hospital,Huludao 125000,China)

机构地区：[1]葫芦岛市中心医院,125000

出　　处：《中国实用医药》2024年第4期78-81,共4页China Practical Medicine

摘　　要：目的探析爱泼斯坦-巴尔病毒(EB病毒,EBV)免疫球蛋白(Ig)M、IgG及脱氧核糖核酸(DNA)检测在儿童呼吸道感染性疾病中的诊断价值。方法选取400例呼吸道感染性疾病患儿作为观察组,同时选取100例无呼吸道感染患儿作为对照组。对两组患儿EBV-IgM、EBV-IgG及EBV-DNA进行检测。比较观察组不同年龄段患儿EBV-IgM、EBV-IgG及EBV-DNA阳性率,两组EBV-IgM、EBV-IgG及EBV-DNA检测结果;分析各项指标的诊断价值。结果观察组不同年龄段患儿EBV-IgM、EBV-IgG阳性率比较,差异具有统计学意义(P<0.05);观察组不同年龄段患儿EBV-DNA阳性率比较无明显差异(P>0.05)。EBV-IgM检测中,观察组化学发光值(11.39±2.12)高于对照组的(9.54±1.35),阳性率50.50%(202/400)高于对照组的5.00%(5/100),差异有统计学意义(P<0.05);EBV-IgG检测中,观察组吸光度值(11.33±2.15)高于对照组的(9.03±1.06),阳性率58.00%(232/400)高于对照组的12.00%(12/100),差异有统计学意义(P<0.05);EBV-DNA检测中,观察组循环数Ct值(34.13±4.88)低于对照组的(43.75±11.62),阳性率57.00%(228/400)高于对照组的6.00%(6/100),差异均具有统计学意义(P<0.05)。EBV-DNA对呼吸道感染性疾病患儿的诊断价值最高[曲线下面积(AUC)=0.644],其次依次是EBV-IgM(AUC=0.618)、EBV-IgG(AUC=0.584),EBV-IgM对呼吸道感染性疾病患儿的诊断特异度最高,为96.12%。结论EBV-IgM、EBV-IgG、EBV-DNA检测在儿童呼吸道感染性疾病的诊断中具有重要的指导意义。Objective To explore the diagnostic value of Epstein-Barr virus(EBV)immunoglobulin(Ig)M,IgG and DNA in children with respiratory infectious diseases.Methods 400 children with respiratory infectious diseases were selected as the observation group and 100 children without respiratory tract infection as the control group.EBV-IgM,EBV-IgG and EBV-DNA were detected in the two groups.The positive rates of EBV-IgM,EBV-IgG and EBV-DNA in children of different ages in the observation group were compared,as well as the detection results of EBV-IgM,EBV-IgG and EBV-DNA between the two groups.The diagnostic value of each index was analyzed.Results The positive rates of EBV-IgM and EBV-IgG in children of different age groups in the observation group were compared,and the difference was statistically significant(P<0.05).There was no significant difference in the positive rate of EBV-DNA in children of different age groups in the observation group(P>0.05).In EBV-IgM detection,the chemiluminescence value of(11.39±2.12)in the observation group was higher than(9.54±1.35)in the control group,and the positive rate of 50.50%(202/400)was higher than 5.00%(5/100)in the control group.The difference was statistically significant(P<0.05).In EBV-IgG detection,the absorbance value of(11.33±2.15)in the observation group was higher than(9.03±1.06)in the control group,and the positive rate of 58.00%(232/400)was higher than 12.00%(12/100)in the control group.The difference was statistically significant(P<0.05).In EBV-DNA detection,the Ct value of cycle number of(34.13±4.88)in the observation group was lower than(43.75±11.62)in the control group,and the positive rate of 57.00%(228/400)was higher than 6.00%(6/100)in the control group.The difference was statistically significant(P<0.05).EBV-DNA has the highest diagnostic value for children with respiratory infectious diseases[area under the curve(AUC)=0.644],followed by EBV-IgM(AUC=0.618),EBV-IgG(AUC=0.584),and EBV-IgM has the highest diagnostic specificity for children with respiratory infect

关 键 词：爱泼斯坦-巴尔病毒 免疫球蛋白M 免疫球蛋白G 脱氧核糖核酸 儿童呼吸道感染性疾病 

分 类 号：R725.6[医药卫生—儿科]