新流腺三联灭活疫苗对禽流感病毒和禽腺病毒流行株攻毒的免疫保护试验  

Protective Test of NDV,AIV and FAdV Inactivated Vaccine against Challenge of Prevalent AIV and FAdV strains

作　　者：杨灵芝 李本科 朱杰 李振清[4] 李桂芹[4] YANG Ling-zhi;LI Ben-ke;ZHU Jie;LI Zhen-qing;LI Gui-qin(Shandong Bolaiwei Biological Technology Institute,Binzhou,Shandong,256606,China;Shandong Binzhou Wohua Biotech Co.,Ltd.,Binzhou,Shandong,256606,China;Binzhou Agricultural Technology Extension Center,Binzhou,Shandong,256600,China;Shandong Binzhou Polytechnic,Binzhou,Shandong,256603,China)

机构地区：[1]山东博莱威生物技术研究所,山东滨州256606 [2]山东滨州沃华生物工程有限公司,山东滨州256606 [3]滨州市农业技术推广中心,山东滨州256600 [4]滨州职业学院,山东滨州256603

出　　处：《动物医学进展》2024年第2期90-94,共5页Progress In Veterinary Medicine

摘　　要：为了验证鸡新城疫、禽流感(H9亚型)、禽腺病毒病(Ⅰ群,4型)三联灭活疫苗对H9亚型禽流感、禽腺病毒(Ⅰ群,4型)流行毒株的免疫保护效果,采用三联灭活疫苗免疫3周龄SPF鸡,免疫后21 d采血测定禽流感H9亚型HI抗体效价,同时分别用H9亚型禽流感病毒YT株、HD株、CX株和DL株以及禽腺病毒(Ⅰ群,4型)QD株、NJ株、HA株和CZ株进行攻毒,观察疫苗对H9亚型禽流感及禽腺病毒(Ⅰ群,4型)流行毒株的免疫保护效果。结果显示,免疫组禽流感H9亚型HI抗体效价几何平均值为1∶676~1∶832,对照组试验鸡HI抗体均<1∶2;用H9亚型禽流感病毒YT株、HD株、CX株和DL株攻毒,对照组鸡9/10~10/10发病,免疫组鸡均9/10以上保护;用禽腺病毒(Ⅰ群,4型)QD株、NJ株、HA株和CZ株攻毒,对照组鸡9/10~10/10发病,免疫组鸡均10/10保护。表明新流腺三联灭活疫苗对2020-2022年分离的H9亚型禽流感病毒及禽腺病毒(Ⅰ群,4型)流行毒株感染具有良好的免疫保护效果。In order to verify the protective efficacy of Newcastle disease virus(NDV),avian influenza virus AIV(subtype H9)and fowl adenovirus(FAdV)(groupⅠ,serotype 4)inactivated vaccine against prevalent AIV(subtype H9)and FAdV(groupⅠ,serotype 4)strains,the triple inactivated vaccine was used to vaccinate specific pathogen-free chickens of 3-week-old.21 days after vaccination,the blood samples were collected and the HI antibody titer of AIV(subtype H9)was detected.After the blood samples were collected,the chickens were challenged with AIV(subtype H9)(YT strain,HD strain,CX strain and DL strain)and FAdV(groupⅠ,serotype 4)(QD strain,NJ strain,HA strain and CZ strain)respectively.A continuous observation of the inactivated vaccine efficacy was performed.The results showed that the geometric mean titer of HI antibodies of AIV(subtype H9)was 1∶676 to 1∶832 in immunized group,while below 1∶2 in control group;The challenge experiment revealed that more than 9/10 of vaccinated chickens were protected,but a morbidity ranged from 9/10 to 10/10 in the control group after challenge with AIV(subtype H9),and 10/10 of vaccinated chickens were protected,but a morbidity ranged from 9/10 to 10/10 in the control group after challenge with FAdV(groupⅠ,serotype 4).The test indicated that the triple inactivated vaccine had good protective efficacy against the prevalent AIV(subtype H9)and FAdV(groupⅠ,serotype 4)strains isolated from 2020 to 2022.

关 键 词：鸡 三联灭活疫苗 流行毒株 免疫 保护 

分 类 号：S852.4[农业科学—基础兽医学] S852.65[农业科学—兽医学]