辛芷通窍颗粒对慢性鼻窦炎小鼠鼻窦黏膜的保护机制研究  

Protective effects and mechanisms of Xinzhi Tongqiao granules on nasal sinus mucosa in chronic rhinosinusitis mice model

作　　者：李群真 李军 郭树繁[1] 燕茹[1] 董学娜 马凤梅[1] LI Qunzhen;LI Jun;GUO Shufan;YAN Ru;DONG Xuena;MA Fengmei(Department of Otolaryngology,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050011,China)

机构地区：[1]河北省中医院耳鼻喉科,河北石家庄050011

出　　处：《陕西中医》2024年第3期318-322,共5页Shaanxi Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局科研计划项目(2018036)。

摘　　要：目的:探讨辛芷通窍颗粒对慢性鼻窦炎(CRS)小鼠鼻窦黏膜的保护作用及相关机制。方法:将50只C57BL/6J小鼠随机分为假手术组、模型组、辛芷通窍低剂量组、辛芷通窍中剂量组、辛芷通窍高剂量组。采用开放上颌窦后接种3型荚膜型肺炎链球菌的方法建立CRS小鼠模型。辛芷通窍低剂量组予以1.4 g/kg辛芷通窍颗粒灌胃、辛芷通窍中剂量组予以2.8 g/kg辛芷通窍颗粒灌胃、辛芷通窍高剂量组予以5.6 g/kg辛芷通窍颗粒灌胃治疗。利用ELISA法检测小鼠外周血炎症因子水平;采用RT-qPCR、Western blot法检测鼻窦黏膜中相关因子表达。结果:与假手术组相比,辛芷通窍低剂量组、辛芷通窍中剂量组、辛芷通窍高剂量组小鼠外周血炎症因子水平明显低于给药前,且窦黏膜组织中TNF-α、IL-6、IL-10、IL-16、IFN-γ的mRNA及蛋白相对表达低于模型组,差异有统计学意义(均P<0.05)。与模型组相比,辛芷通窍低剂量组、辛芷通窍中剂量组、辛芷通窍高剂量组小鼠鼻窦黏膜组织中HMGB1、NLRP3、ASC、Cleaved caspase-1蛋白表达降低,差异有统计学意义(均P<0.05)。结论:辛芷通窍颗粒呈剂量依赖性地抑制HMGB1/NLRP3信号通路激活,抑制CRS小鼠鼻窦黏膜炎症水平。Objective:To explore protective effect and mechanism of Xinzhi Tongqiao granules on nasal sinus mucosa of chronic rhinosinusitis(CRS)mice.Methods:Fifty C57BL/6J mice were randomly divided into sham operation group,model group,low-dose group of Xinzhi Tongqiao,medium-dose groupof Xinzhi Tongqiao,and high-dose group of Xinzhi Tongqiao.The CRS mouse model were established by intranasal instillation of three types of encapsulated pneumococci afteropen maxillary sinus surgery.The low-dose group of Xinzhi Tongqiao were treated with 1.4 g/kg of Xinzhi Tongqiao granules,the medium-dose group of Xinzhi Tongqiao with 2.8 g/kg of Xinzhi Tongqiao granules,and the high-dose group of Xinzhi Tongqiao with 5.6 g/kg of Xinzhi Tongqiao granules.The expression levels of inflammatory factors in peripheral blood and relevant factors in nasal sinus mucosa were measured by RT-qPCRWestern blot.Results:Compared with the sham operation group,the levels of inflammatory factors in the peripheral blood of the low-dose group of Xinzhi Tongqiao,the medium-dose group of Xinzhi Tongqiao,and the high-dose group of Xinzhi Tongqiao were significantly lowerthan those before administration,and the relative expression levels of TNF-α,IL-6,IL-10,IL-16,and IFN-γmRNA and protein in the sinus mucosa were significantly lowerthan those in the model group,difference statistically significant(all P<0.05).Compared with the model group,the expression levels of HMGB1,NLRP3,ASC,and Cleaved caspase-1 proteins in the nasal sinus mucosa of the low-dose group of Xinzhi Tongqiao,the medium-dose group of Xinzhi Tongqiao,and the high-dose group of Xinzhi Tongqiao were lower,difference statistically significant(all P<0.05).Conclusion:Xinzhi Tongqiao granules can dose-dependently inhibit activation of HMGB1/NLRP3 signaling pathway and suppress level of nasal sinus mucosal inflammation in CRS mice.

关 键 词：慢性鼻窦炎 辛芷通窍颗粒 胞内高迁移率族蛋白B1 NOD样受体蛋白3 炎症因子 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]