基于拉曼频移的SERS分析平台在胃癌早期诊断中的应用研究  

Raman frequency shift-based SERS analysis platform for early diagnosis of gastric cancer

作　　者：韦伟 朱群山 黄永 石立新 吴正 杨永国 刘佳 袁梦轩 王震光 蒋丰娟 WEI Wei;ZHU Qunshan;HUANG Yong;SHI Lixin;WU Zheng;YANG Yongguo;LIU Jia;YUAN Mengxuan;WANG Zhenguang;JIANG Fengjuan(Department of Gastrointestinal Surgery,Jiangdu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225200,China;Department of Gastroenterology,Jiangdu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225200,China;Department of General Surgery,Jiangdu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225200,China;Department of Pathology,Jiangdu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225200,China;Department of Radi-ology,Jiangdu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225200,China;Department of Clinical Laboratory,Jiangdu People’s Hospital Affiliated to Yangzhou University,Yangzhou 225200,China)

机构地区：[1]扬州大学附属江都人民医院胃肠外科,扬州225200 [2]扬州大学附属江都人民医院消化内科,扬州225200 [3]扬州大学附属江都人民医院普通外科,扬州225200 [4]扬州大学附属江都人民医院病理科,扬州225200 [5]扬州大学附属江都人民医院放射科,扬州225200 [6]扬州大学附属江都人民医院检验科,扬州225200

出　　处：《分析试验室》2024年第1期9-17,共9页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(81701825);扬州市社会发展基金(YZ2022089)资助。

摘　　要：利用界面自组装方法制备了具有优良均一性、稳定性和SERS活性的金银纳米梭(Au-AgNSs)基底。通过将4-巯基苯甲酸(4-MBA)修饰在Au-AgNSs基底表面,并结合癌胚抗原(CEA)的单克隆抗体,实现了Au-AgNSs基底的功能化。血清样本中CEA可被其抗体捕获并使4-MBA发生形变,导致特征峰发生偏移。经验证,该基于拉曼频移的SERS分析平台具有优异的特异性、重复性和灵敏度,对血清中CEA的检测限(LOD)低至1.22 pg/mL。利用其对胃癌(GC)患者和健康受试者血清中CEA的表达水平进行测定,并与酶联免疫吸附测定法(ELISA)结果比较。结果表明,GC患者血清CEA表达水平高于健康受试者,且2种方法结果无明显差异。因此基于拉曼频移的SERS分析平台在GC诊断中具有应用价值。Au-AgNSs substrates with excellent uniformity,stability and SERS activity were prepared using an interface self-assembly strategy.The functionalization of Au-AgNSs substrates was achieved by modifying 4-mercaptobenzoic acid(4-MBA) onto the surface of Au-AgNSs substrates and binding the anti-CEA.CEA in serum samples can be captured by anti-CEA and deforms 4-MBA,resulting in a shift of the characteristic peak.The Raman frequency shift-based SERS platform was validated to have excellent specificity,reproducibility,and sensitivity,with the limit of detection(LOD) as low as 1.22 pg/mL for CEA in serum.The SERS analysis platform was applied for the determination of CEA expression levels in gastric cancer(GC) patients and healthy human,and compared with ELISA method.The results showed that the expression levels of CEA in GC patients were higher than those of healthy human,and there was no significant difference between the two methods.Therefore,the SERS platform based on Raman frequency shift is valuable in the diagnosis of GC.

关 键 词：拉曼频移 表面增强拉曼散射 胃癌 早期诊断 生物标志物 

分 类 号：O657.37[理学—分析化学]