基于金纳米粒子探针比色法检测乙基谷硫磷  

Colorimetric detection of ethyazinphos-methyl based on gold nanoparticle probes

作　　者：张亚莉[1] 田璐肖 徐鑫林 王未肖[1] 哈婧[2] ZHANG Yali;TIAN Luxiao;XU Xinlin;WANG Weixiao;HA Jing(College of Sciences,Hebei University of Science and Technology,Shijiazhuang 050018,China;College of Chemical&Pharmacetical Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)

机构地区：[1]河北科技大学理学院,石家庄050018 [2]河北科技大学化学与制药工程学院,石家庄050018

出　　处：《分析试验室》2024年第1期86-90,共5页Chinese Journal of Analysis Laboratory

基　　金：科技部重大新药创制专项(2019ZX09302-047);河北省级研究生示范课程建设项目(KCJSX2021073);河北科技大学教育教学改革研究与实践项目(2020-ZD06)资助。

摘　　要：基于乙基谷硫磷在酸性条件下可以诱导金纳米粒子(AuNPs)发生聚集,建立了以AuNPs为探针、结合比色和分光光度法检测乙基谷硫磷的方法。通过改变氯金酸和还原剂柠檬酸钠的比例,制备了不同粒径的AuNPs。酸性溶液中乙基谷硫磷分子中-P=S键发生质子化,形成的-SH与Au形成S-Au键,使AuNPs发生聚集,溶液颜色由红色转变为蓝色。考察了乙酸-乙酸钠缓冲溶液的pH和浓度以及乙基谷硫磷与AuNPs的作用时间对AuNPs聚集程度的影响。在优化条件下,吸光度比值(A694 nm/A524 nm)与乙基谷硫磷浓度在0.392~0.603μmol/L范围内具有良好的线性关系,检出限为0.0782μmol/L。Based on the aggregation of gold nanoparticles(AuNPs) induced by ethyazinphos-methyl under acidic conditions,a new method for the detection of ethyazinphos-methyl using AuNPs as a probe combined with colorimetric and Uv-visible spectrophotometry was developed.AuNPs of different sizes were prepared by varying the ratio of sodium citrate to chloroauric acid.The-P=S bond was protonated in ethyl azinphos-methyl in acidic solution,the produced-SH formed an S-Au bond with Au,causing AuNPs aggregation and the color of the solution changed from red to blue.The effects of pH and concentration of acetic acid-sodium acetate buffer solution,and the interaction time between ethyazinphos-methyl and AuNPs were investigated.There was a good linear relationship between the absorbance ratio(A694 nm/A524 nm) and the concentration of ethyl azinphos-methyl in the range of 0.392-0.603 μmol/L,and the detection limit was 0.0782 μmol/L.

关 键 词：金纳米粒子 乙基谷硫磷 比色法 分光光度法 聚集 

分 类 号：TQ131[化学工程—无机化工] G642[文化科学—高等教育学]