绿原酸和咖啡酸对雷州山羊精液冷冻保存效果的影响  

Effects of chlorogenic and caffeic acids on the cryopreservation ofsemen from Leizhou goats

作　　者：苗丰帅 李靖 蒋欣怡 陈宣旭 于海滨 姜平 林紫薇 赵志辉 照那木拉 MIAO Fengshuai;LI Jing;JIANG Xinyi;CHEN Xuanxu;YU Haibin;JIANG Ping;LIN Ziwei;ZHAO Zhihui;Zhaonamula(College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学滨海农业学院,广东湛江524088

出　　处：《中国兽医科学》2024年第1期134-140,共7页Chinese Veterinary Science

基　　金：广东省重点领域研发计划项目(2022B0202110002);广东省乡村振兴战略专项(2022-XPY-00-010);广东省自然科学基金项目(2021A1515010867);粤西地区畜禽资源与种质创新重点实验室基金项目(2021A1515010867);广东海洋大学科研启动基金项目(R17032,060302052315)。

摘　　要：为了提高冻-解后精液品质,探讨绿原酸和咖啡酸对雷州山羊精液冷冻保存效果的影响,笔者在冷冻保存稀释液中分别添加100μmol/L的绿原酸(CGA)和咖啡酸(CA),并以无添加组为对照,实施了冷冻保存实验。通过检测冻-解后精子活率、活力、生存率、质膜完整性、顶体完整性等指标评估精液品质,并检测了活性氧(ROS)水平、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、氧化产物丙二醛(MDA)、线粒体膜电位(MMP)等抗氧化指标。结果显示,在冷冻保存稀释液中添加CGA和CA可显著(P<0.05)提高冻-解雷州山羊精液品质(精子活率、活力、生存率等),有效提高SOD、GSH-PX、CAT等酶活性和MMP(P<0.05),降低了精子ROS水平和MDA的含量(P<0.05),从而提高了雷州山羊精液冷冻保存效果。结果表明,在冷冻保存稀释液中添加CGA和CA能显著提高冻-解后雷州山羊精液品质和抗氧化性能,进而提高雷州山羊精液冷冻保存效果。To improve the quality of frozen-thawed semen,this study investigated the effects of chlorogenic acid(CGA)and caffeic acid(CA)on the cryopreservation of semen from Leizhou goats.100μmol/L CGA and CA were added to the freezing dilution solution,respectively.The group without addition served as the control,and cryopreservation experiment was conducted.The semen quality was evaluated by assessing frozen-thawed sperm viability,motility,survival rate,plasma membrane integrity,acrosome integrity,and antioxidant indicators such as reactive oxygen species(ROS)level,catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),and mitochondrial membrane potential(MMP).The results showed that the addition of CGA and CA in the cryopreservation diluent significantly improved frozen-thawed semen quality(sperm motility,viability,survival rate,etc.)of Leizhou goats(P<0.05).The enzyme activities of SOD,GSH-PX,CAT and MMP were effectively increased(P<0.05),and the sperm ROS level and MDA content were decreased(P<0.05),so the cryopreservation effect of Lezhou goats semen was improved.The research results indicate that the quality and antioxidant properties of Leizhou goats semen are significantly improved after frozen-thawed by adding CGA and CA to the frozen preservation diluent,thereby enhancing the cryopreservation effect of Leizhou goats semen.

关 键 词：绿原酸 咖啡酸 雷州山羊 精液冷冻保存 氧化应激 

分 类 号：S821.34[农业科学—畜牧学]