右美托咪定对IL-1β诱导的人髓核细胞凋亡和炎症反应的影响  

Effects of dexmedetomidine on IL-1β-induced apoptosis and inflammatory response of human nucleus pulposus cells

作　　者：王磊 吴海龙 张金强 WANG Lei;WU Hailong;ZHANG Jinqiang(Department of Anesthesiology,the PLA Rocket Force Characteristic Medical Center,Beijing 102209,China)

机构地区：[1]中国人民解放军火箭军特色医学中心麻醉科,北京102209

出　　处：《山东医药》2024年第7期52-55,共4页Shandong Medical Journal

摘　　要：目的观察右美托咪定(Dex)对白细胞介素1β(IL-1β)诱导的人髓核细胞凋亡和炎症反应的影响。方法常规培养人髓核细胞,并将其随机分为对照组(Control组)、IL-1β组、IL-1β+Dex-L组、IL-1β+Dex-M组、IL-1β+Dex-H组。Control组不做任何处理,其他组依次给予10 ng/mL IL-1β、10 ng/mL IL-1β+25 nmol/L Dex、10 ng/mL IL-1β+50 nmol/L Dex、10 ng/mL IL-1β+100 nmol/L Dex,继续培养24 h。用MTT法检测细胞存活率,流式细胞术检测细胞凋亡率,酶联免疫吸附法检测细胞上清液中肿瘤坏死因子α(TNF-α)、白细胞介素8(IL-8)、一氧化氮(NO)、白细胞介素6(IL-6),Western blotting法检测B细胞淋巴瘤/白血病2(Bcl-2)、Bcl-2相关X蛋白(Bax)、诱导型一氧化氮合成酶(iNOS)、环氧化酶2(COX-2)蛋白。结果与Control组比较,IL-1β组细胞培养24、48 h存活率及Bcl-2蛋白表达低,凋亡率、Bax蛋白表达、TNF-α、IL-6、IL-8及COX-2、iNOS蛋白表达、NO水平高,差异均有统计学意义(P均<0.05);与IL-1β组比较,IL-1β+Dex-L组、IL-1β+Dex-M组、IL-1β+Dex-H组细胞培养24、48 h存活率及Bcl-2蛋白表达高,凋亡率、Bax蛋白表达、TNF-α、IL-6、IL-8及COX-2、iNOS蛋白表达、NO水平低,差异均有统计学意义(P均<0.05);IL-1β+Dex-L组、IL-1β+Dex-M组、IL-1β+Dex-H组细胞培养24、48 h存活率及Bcl-2蛋白表达依次升高,凋亡率、Bax蛋白表达、TNF-α、IL-6、IL-8及COX-2、iNOS蛋白表达、NO水平依次降低,差异均有统计学意义(P均<0.05)。结论右美托咪定可抑制IL-1β诱导的人髓核细胞凋亡,减轻炎症反应,且该作用呈浓度依赖性。Objective To observe the effects of dexmedetomidine(Dex)on apoptosis and inflammatory response of human nucleus pulposus cells induced by interleukin-1β(IL-1β).Methods Human nucleus pulpocytes were cultured in vitro and divided into the Control group(without any treatment),IL-1βgroup(cultured with 10 ng/mL IL-1β for 24 h),IL-1β+Dex-L group(cultured with 25 nmol/L dexmedetomidine and 10 ng/mL IL-1β for 24 h),IL-1β+Dex-M group(cultured with 50 nmol/L dexmedetomidine and 10 ng/mL IL-1βfor 24 h),and IL-1β+Dex-H group(cultured with 100 nmol/L dexmedetomidine and 10 ng/mL IL-1β for 24 h),respectively.The cell survival rate was measured by MTT,the apoptosis rate was detected by flow cytometry,and the tumor necrosis factor-α(TNF-α),interleukin-8(IL-8),nitric oxide(NO)and interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay(ELISA).The expression levels of B-cell lymphoma/leukemia 2(Bcl-2),Bcl-2 associated X protein(Bax),inducible-nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)were detected by Western blotting.Results Compared with the Control group,the cell survival rates at 24 h and 48 h and Bax protein expression in the IL-1β group decreased,while the apoptosis rate,Bax protein expression,TNF-α,IL-6,IL-8,COX-2,iNOS protein expression and NO expression increased,with statistically significant difference(all P<0.05).Compared with the IL-1β group,the survival rates of cells at 24 h and 48 h were higher,and the expression of Bcl-2 protein was higher,the apoptotic rate,the expression levels of Bax protein,TNF-α,IL-6,IL-8,COX-2,iNOS protein,and NO were lower in the IL-1β+Dex-L group,IL-1β+Dex-M group,and IL-1β+Dex-H group,and the differences were all statistically significant(all P<0.05).The survival rates of cells at 24 h and 48 h and the expression of Bcl-2 protein increased successively,the apoptotic rate,the expression levels of Bax protein,TNF-α,IL-6,IL-8,COX-2,iNOS protein,and NO decreased successively in the IL-1β+Dex-L group,IL-1β+Dex-M group,and IL-1β+Dex-H group,and the

关 键 词：椎间盘退变 右美托咪定 白细胞介素1Β 髓核细胞 细胞凋亡 炎症反应 

分 类 号：R681.5[医药卫生—骨科学]