Improving anammox activity and reactor start-up speed by using CO_(2)/NaHCO_(3) buffer  

作　　者：Shaoan Cheng Huahua Li Xinyuan He Hua Chen Longxin Li 

机构地区：[1]State Key Laboratory of Clean Energy Utilization,College of Energy Engineering,Zhejiang University,Hangzhou 310027,China

出　　处：《Journal of Environmental Sciences》2024年第5期60-71,共12页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.52070162)。

摘　　要：Anammox bacteria grow slowly and can be affected by large pH fluctuations.Using suitable buffers could make the start-up of anammox reactors easy and rapid.In this study,the effects of three kinds of buffers on the nitrogen removal and growth characteristics of anammox sludge were investigated.Reactors with CO_(2)/NaHCO_(3)buffer solution(CCBS)performed the best in nitrogen removal,while 4-(2-hydroxyerhyl)piperazine-1-ethanesulfonic acid(HEPES)and phosphate buffer solution(PBS)inhibited the anammox activity.Reactors with 50 mmol/L CCBS could start up in 20 days,showing the specific anammox activity and anammox activity of 1.01±0.10 gN/(gVSS·day)and 0.83±0.06 kgN/(m^(3)·day),respectively.Candidatus Kuenenia was the dominant anammox bacteria,with a relative abundance of 71.8%.Notably,anammox reactors could also start quickly by using 50 mmol/L CCBS under nonstrict anaerobic conditions.These findings are meaningful for the quick start-up of engineered anammox reactors and prompt enrichment of anammox bacteria.

关 键 词：Anammox bacteria Buffer solution CO_(2)/NaHCO_(3) Physiological characteristics Microbial community shift 

分 类 号：X703[环境科学与工程—环境工程]