盐胁迫下中国沙棘生理指标变化及水通道蛋白基因的表达模式  

Physiology and Expression of PIP of Hippophae rhamnoides under Salt Stress

作　　者：施会 严雯雯 尹代浩 张宇 王蔚 鲍玉英 马玉花[1] SHI Hui;YAN Wenwen;YIN Daihao;ZHANG Yu;WANG Wei;BAO Yuying;MA Yuhua(College of Agriculture and Husbandry,Qinghai University,Xining,Qinghai 810016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016

出　　处：《福建农业学报》2023年第12期1414-1419,共6页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31660071);青海省科技厅项目(2017-ZJ-734)。

摘　　要：【目的】探讨水通道蛋白(Plasma membrane intrinsic proteins,PIPs)在中国沙棘盐胁迫响应中的作用,为中国沙棘应对盐胁迫机理研究提供参考。【方法】以中国沙棘(Hippophae rhamnoides subsp. sinensis)为试验材料,对中国沙棘质膜内在蛋白基因(HrPIP)序列进行生物信息学分析,并研究不同程度盐胁迫(200、400、600、800、1 000mmol·L^(-1))对中国沙棘生理指标及HrPIP基因表达模式的影响。【结果】中国沙棘HrPIP基因编码110个氨基酸,其编码蛋白质位于细胞膜上,无信号肽,无跨膜螺旋区,为疏水性蛋白。中国沙棘相对含水量随着盐胁迫的加深逐渐下降;叶绿素含量总体呈先下降后升高的趋势;质膜透性和丙二醛含量在低浓度盐胁迫下变化不大,在高盐浓度下急剧上升,在1 000 mmol·L^(-1)时丙二醛含量有所降低而膜透性继续升高。HrPIP基因的表达随着盐胁迫程度的加剧而变化,其在根中的表达量呈先上升后下降再上升的趋势,在叶中呈先下调后急剧上升而后又下降的趋势,在茎中则呈“M”型表达模式。【结论】中国沙棘在盐胁迫下具有一定的抗盐性,胁迫下HrPIP基因的表达量变化促进根的吸水、茎的运输、叶的保水等,从而提高水分吸收,调节自身的耐盐性,说明该基因在中国沙棘应对盐胁迫的过程中起着重要作用。【Objective】Mechanism of aquaporins relating to the plasma membrane intrinsic proteins(PIPs)of Hippophae rhamnoides subsp.sinensis in response to salt stress was studied.【Methods】Bioinformatics of HrPIP was analyzed.Physiological indexes and expressions of the gene in tissues of H.rhamnoides under normal and imposed salt stresses of 200,400,600,800,or 1,000 mmol·L^(-1) NaCl were determined.【Results】Located in the cell membrane,HrPIP encoded 110 amino acids.The hydrophobic protein had no signal peptide or transmembrane helix region.As salt stress intensified,the RWC decreased gradually,and the chlorophyll content declined initially followed by an incline.The permeability of the plasma membrane and the content of MDA changed slightly when the salt concentration was low but increased significantly when the concentration was high.The 1000 mmol·L^(-1) salt stress induced declined MDA but continuously rising membrane permeability.The expressions of HrPIP in different organs varied by the increasing salt stress.In roots,it rose at lower salt concentrations then declined but rose again at high salt levels.In the leaves,the opposite trend was observed,whereas it was in an“M”pattern in the stems.【Conclusion】H.rhamnoides subsp.sinensis was salt resistant to a certain degree.Under the stress,by altering the HrPIP expression to increase water absorption in roots,transport in stems,and retention in leaves the plant manipulated the cellular salt concentration to achieve an improved stress tolerance.

关 键 词：中国沙棘 HrPIP基因 盐胁迫 表达模式 

分 类 号：S793.6[农业科学—林木遗传育种]