不同抗性水稻品种响应纹枯病菌侵染的代谢组差异分析  

Analysis of metabolomic differences in the response of different resistant rice varieties to sheath blight inoculation

作　　者：卢帅兵 谢文亚 岳轩宇 李梦臣 冯志明 陈宗祥[1,2] 胡珂鸣 陈夕军 曹文磊[4] 左示敏 LU Shuaibing;XIE Wenya;YUE Xuanyu;LI Mengchen;FENG Zhiming;CHEN Zongxiang;HU Keming;CHEN Xijun;CAO Wenlei;ZUO Shimin(Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of Ministry of Education/College of Agriculture,Yangzhou University,Yangzhou 225009,China;Jiangsu Collaborative Innovation Center for Modern Industrial Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,China;College of Plant Protection,Yangzhou University,Yangzhou 225009,China;College of Tourism and Culinary,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学农学院/江苏省作物基因组学和分子育种重点实验室/生物育种钟山实验室/植物功能基因组学教育部重点实验室,江苏扬州225009 [2]扬州大学江苏省粮食作物现代产业技术协同创新中心/江苏省作物遗传生理重点实验室,江苏扬州225009 [3]扬州大学植物保护学院,江苏扬州225009 [4]扬州大学旅游烹饪学院,江苏扬州225009 [5]扬州大学教育部农业与农产品安全国际合作联合实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2023年第6期9-17,25,共10页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(32200430、32000362);江苏省自然科学基金资助项目(BK20200930);上海市2023年度“科技创新行动计划”农业科技领域项目(23N11900100);中国博士后科学基金资助项目(2022M712700);江苏高校优势学科建设工程项目(PAPD)。

摘　　要：利用气相色谱-飞行时间串联质谱(GC/TOF-MS)检测抗纹枯病品种(YSBR1)YS和感纹枯病品种(Lemont)LE接种纹枯病菌(Rhizoctonia solani Kühn)后的代谢组差异,以探究水稻抗纹枯病机制。结果表明:R.solani侵染后,YS和LE分别有82和75个差异代谢物(DAM)的含量升高;相对于LE,YS中存在更多的氨基酸类差异代谢物,其中合成抗病因子(水杨酸、乙烯、木质素、叶绿素和γ-氨基丁酸)的氨基酸及其衍生物受诱导水平明显高于LE。结果提示YS对纹枯病的较强抗性可能与其自身特有的代谢产物尤其是抗病因子相关的氨基酸及其衍生物密切相关。Gas chromatography-time-of-flight tandem mass spectrometry(GC/TOF-MS) was used to detect metabolomic differences between the disease-resistant variety YSBR1(YS) and the disease-susceptible variety Lemont(LE) after inoculation with Rhizoctonia solani Kühn in order to investigate the mechanism of rice resistance to sheath blight.The results showed that after the inoculation of R.solani,YS and LE elevated to respectively 82 and 75 differential metabolites(DAM)levels.More amino acid-like differential metabolites were present in YS compared to LE,the induced levels of amino acids and their derivatives synthesize disease resistance factors(salicylic acid,ethylene,lignin,chlorophyll andγ-aminobutyric acid)in YS were significantly higher than those in LE.The results suggested that the higher resistance of YS to R.solani may be closely related to its specific metabolites,especially amino acids and their derivatives related to disease resistance factors.

关 键 词：水稻 纹枯病 抗性差异 代谢组 

分 类 号：S511[农业科学—作物学]