不同产地牡丹皮薄层鉴别及丹皮酚含量测定  

Thin Layer Identification and Determination of Paeonol Content in Moutan Cortex from Different Origins

作　　者：殷宏艳 尤斯涵 赵永明[1] 颜凡莉 陈明霞 姜海燕 郭春燕[1] YIN Hong-yan;YOU Si-han;ZHAO Yong-ming;YAN Fan-li;CHEN Ming-xia;JIANG Hai-yan;GUO Chun-yan(Department of Pharmacy,Hebei North University,Hebei Key Laboratory of Neuropharmacology,Zhangjia kou,075000,China;Beijing Silien Pharmaceutical Co.,Beijing,100176,China)

机构地区：[1]河北北方学院药学院,河北省神经药理学重点实验室,张家口075000 [2]北京斯利安药业有限公司,北京100176

出　　处：《神经药理学报》2023年第5期24-28,共5页Acta Neuropharmacologica

基　　金：河北省教育厅创新创业教育教学改革研究与实践项目(No.2023cxcy159)。

摘　　要：目的:高效液相色谱法测定安徽、河南、山东产地牡丹皮中丹皮酚含量,薄层色谱鉴别不同产地牡丹皮药材。方法:采用G型薄层层析硅胶薄层板,以环己烷-乙酸乙酯-冰醋酸(4∶1∶0.1)为展开剂,喷以2%香草酸硫酸乙醇溶液显色;采用喆分C18色谱柱(4.6 mm×250 mm, 5μm),流动相为甲醇-水(45∶55 V/V)洗脱,流速为1.0 mL·min^(-1),检测波长为274 nm,柱温为30℃,进样体积为10μL。结果:21批次牡丹皮药材薄层鉴别斑点显色清晰,丹皮酚含量的范围为2.7%~2.9%,均高于药典标准,其中山东省丹皮酚含量最高。结论:山东、河南、安徽种植的牡丹皮质量好,品相佳,不同批次间差异小,品质均匀稳定。Objective:High performance liquid chromatography(HPLC)was used to determine the content of paeonol in Moutan Cortex of different origins,and thin-layer chromatography(TLC)was used to identify the herbs of Moutan Cortex of different origins.Methods:The separation was performed on a G-type thin-layer chromatography silica gel plate with cyclohexane-ethyl acetate-glacial acetic acid(4:1:0.1)as the unfolding agent,and the color was developed by spraying with 2%vanillic acid ethanol sulfate solution;the separation was carried out on a Chevonne C18 column(4.6 mm X250 mm,5μm)with the elution of methanol-water(45:55 V/V)as the mobile phase,at the flow rate of 1.O mL·min^(-1) and the detection wavelength of 274 nm.The mobile phase was methanol-water(45:55 V/V)at a flow rate of 1.0 mL·min^(-1) ,the detection wavelength was 274 nm,the column temperature was 30 C,and the injection volume was 10μL.Results:The results showed that the thinlayer identification spots of 21 batches of peony dandelion herbs were clear,and the contents of dandelion were in the range of 2.7%~2.9%,which were higher than the pharmacopoeia standard,of which dandelion in Shandong province was the highest.Conclusion:The quality of peony bark grown in Shandong,Henan and Anhui is good,with good character,small differences between different batches,and uniform and stable quality.

关 键 词：牡丹皮 高效液相色谱法 薄层色谱法 丹皮酚 

分 类 号：R282[医药卫生—中药学]