Imprinting at the KBTBD6 locus involves species-specific m ternal methylation and monoallelic expression in livestock animals  

作　　者：Jinsoo Ahn In-Sul Hwang Mi-Ryung Park Seongsoo Hwang Kichoon Lee 

机构地区：[1]Department of Animal Sciences,The Ohio State University,Columbus,OH 43210,USA [2]Animal Biotechnology Division,Rural Development Administration,National Institute of Animal Science,Jeonbuk 55365,Republic of Korea [3]Columbia Center for Translational Immunology,Columbia University Irving Medical Center,Columbia University,New York,NY 10032,USA [4]Animal Welfare Research Team,National Institute of Animal Science,RDA,1500,Kongjwipatjwi‑ro,Jeollabuk‑do 55365,Republic of Korea

出　　处：《Journal of Animal Science and Biotechnology》2024年第1期127-145,共19页畜牧与生物技术杂志（英文版）

基　　金：partially supported by the United States Department of Agriculture National Institute of Food and Agriculture Hatch Grant (Project No.OHO01304)。

摘　　要：Background The primary differentially methylated regions(DMRs) which are maternally hypermethylated serve as imprinting control regions(ICRs) that drive monoallelic gene expression, and these ICRs have been investigated due to their implications in mammalian development. Although a subset of genes has been identified as imprinted, in-depth comparative approach needs to be developed for identification of species-specific imprinted genes. Here, we examined DNA methylation status and allelic expression at the KBTBD6 locus across species and tissues and explored potential mechanisms of imprinting.Results Using whole-genome bisulfite sequencing and RNA-sequencing on parthenogenetic and normal porcine embryos, we identified a maternally hypermethylated DMR between the embryos at the KBTBD6 promoter Cp G island and paternal monoallelic expression of KBTBD6. Also, in analyzed domesticated mammals but not in humans, non-human primates and mice, the KBTBD6 promoter Cp G islands were methylated in oocytes and/or allelically methyl-ated in tissues, and monoallelic KBTBD6 expression was observed, indicating livestock-specific imprinting. Further analysis revealed that these Cp G islands were embedded within transcripts in porcine and bovine oocytes which coexisted with an active transcription mark and DNA methylation, implying the presence of transcription-dependent imprinting.Conclusions In this study, our comparative approach revealed an imprinted expression of the KBTBD6 gene in domesticated mammals, but not in humans, non-human primates, and mice which implicates species-specific evolution of genomic imprinting.

关 键 词：Differentially methylated region Domesticated mammal IMPRINTING KBTBD6 PARTHENOGENETIC 

分 类 号：S813[农业科学—畜牧学]