Dietary Macleaya cordata extract supplementation improves the growth performance and gut health of broiler chickens with necrotic enteritis  

作　　者：Bochen Song Jie He Xue Pan Linglian Kong Chuanpi Xiao Chake Keerqin Zhigang Song 

机构地区：[1]Key Laboratory of Efficient Utilization of Non‑Grain Feed Resources,College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,Shandong,China [2]Center for Mitochondria and Healthy Ageing,College of Life Sciences,Yantai University,Yantai 264005,Shandong,China [3]Precision Livestock and Nutrition Unit,University of Liège,Gembloux Agro‑Bio TechGembloux,Belgium [4]Phytobiotics(Jiangsu)Biotech Co.,Ltd.,Jintan 213200,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第1期374-395,共22页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(32272910);the Shandong Provincial Postdoctoral Program for Innovative Talent(SDBX2021013);the Shandong Province Agricultural Industry Technology(SDAIT-11-08)。

摘　　要：Background The poultry industry needs effective antibiotic alternatives to control outbreaks of necrotic enteritis(NE)caused by Clostridium perfringens.Methods The aim of this study was to investigate the effects of dietary supplementation with Macleaya cordata extract(MCE)on the immune function and gut microbiota of broilers with NE.A total of 2881-day-old broiler chicks were randomly assigned to a 2×2 factorial arrangement with two concentrations of dietary MCE supplementation(0 or 350 mg/kg of diet)and two disease challenge statuses(control or NE).Results The results revealed that NE significantly increased the feed conversion rate(FCR),mortality,intestinal lesion score,the levels of IL-1β,IL-17 and IFN-γ/IL-4 in serum and IL-17/IL-10 in the jejunal mucosa,m RNA levels of TLR2,IFN-γand p Ig R in the jejunum,and Clostridium perfringens concentrations in the cecum.NE significantly decreased the body weight(BW),body weight gain(BWG),jejunal villus height,V/C,m RNA level of AMPK-α1 in jejunum,IL-4 level in the jejunal mucosa and lactic acid bacteria abundance in the cecum.MCE significantly increased BW,BWG,jejunal villus height,V/C,m RNA levels of occludin,ZO-1 and AMPK-α1 in the jejunum,the levels of Ig A and Ig G in serum and IL-10 in the jejunal mucosa and m RNA levels of NF-κB,IL-10 and MHC-II in the jejunum.Additionally,MCE significantly decreased the FCR,mortality,intestinal lesion score,jejunal crypt depth,the levels of IFN-γand IL-17 in serum and IL-17/IL-10 in the jejunal mucosa,Clostridium perfringens concentrations in the cecum,and m RNA levels of IL-17/IL-10 in the jejunum.Moreover,NE significantly increased the abundance of bacteria that are associated with inflammation,obesity and depression(Alistipes,Barnesiella,Intestinimonas,RF39 and UCG-005)and significantly decreased the abundance of short-chain fatty acid(SCFA)-producing bacteria(Anaerotruncus,Butyricicoccus and Bacteroides)in the cecum.MCE significantly increased the abundance of SCFA-producing bacteria(Streptococcus,Ruminococcus_torqu

关 键 词：Broiler chicken Gut microbiota Immune function Macleaya cordata extract Necrotic enteritis 

分 类 号：S831.5[农业科学—畜牧学]