Rumen microbial degradation of bromoform from red seaweed(Asparagopsis taxiformis)and the impact on rumen fermentation and methanogenic archaea  

作　　者：Pedro Romero Alejandro Belanche Elisabeth Jiménez Rafael Hueso Eva Ramos-Morales Joan King Salwen Ermias Kebreab David R.Yáñez-Ruiz 

机构地区：[1]Estacion Experimental del Zaidin,Consejo Superior de Investigaciones Cientificas(CSIC),Profesor Albareda 1,Granada 18008,Spain [2]Department of Animal Production and Food Sciences,University of Zaragoza,Miguel Servet 177,Saragossa 50013,Spain [3]Blue Ocean Barns Inc.,Redwood City,CA,USA [4]Department of Animal Science,University of California,Davis,CA 95618,USA

出　　处：《Journal of Animal Science and Biotechnology》2024年第1期412-426,共15页畜牧与生物技术杂志（英文版）

基　　金：funded by Blue Ocean Barns.AB has a Ramón y Cajal research contract(RYC2019-027764-I)funded by the Spanish State Research Agency(AEI)。

摘　　要：Background The red macroalgae Asparagopsis is an effective methanogenesis inhibitor due to the presence of halogenated methane(CH_(4))analogues,primarily bromoform(CHBr_(3)).This study aimed to investigate the degradation process of CHBr3 from A taxiformis in the rumen and whether this process is diet-dependent.An in vitro batch culture system was used according to a 2×2 factorial design,assessing two A taxiformis inclusion rates[0(CTL)and 2%DM diet(AT)]and two diets[high-concentrate(HC)and high-forage diet(HF)].Incubations lasted for 72 h and samples of headspace and fermentation liquid were taken at 0,0.5,1,3,6,8,12,16,24,48 and 72 h to assess the pattern of degradation of CHBr_(3) into dibromomethane(CH_(2)Br_(2))and fermentation parameters.Additionally,an in vitro experiment with pure cultures of seven methanogens strains(Methanobrevibacter smithii,Methanobrevibacter ruminantium,Methanosphaera stadtmanae,Methanosarcina barkeri,Methanobrevibacter millerae,Methanorhermobacter wolfei and Methanobacterium mobile)was conducted to test the effects of increasing concentrations of CHBr3(0.4,2,10and 50μmol/L).Results The addition of AT significantly decreased CH_(4) production(P=0.002)and the acetate:propionate ratio(P=0.003)during a 72-h incubation.The concentrations of CHBr_(3) showed a rapid decrease with nearly 90%degraded within the first 3 h of incubation.On the contrary,CH_(2)Br_(2) concentration quickly increased during the first 6 h and then gradually decreased towards the end of the incubation.Neither CHBr_(3) degradation nor CH_(2)Br_(2) synthesis were affected by the type of diet used as substrate,suggesting that the fermentation rate is not a driving factor involved in CHBr_(3)degradation.The in vitro culture of methanogens showed a dose-response effect of CHBr3 by inhibiting the growth of M.smithii,M.ruminantium,M.stadtmanae,M.barkeri,M.millerae,M.wolfei,and M.mobile.Conclusions The present work demonstrated that CHBr_(3) from A.taxiformis is quickly degraded to CH_(2)Br_(2)in the rumen and that the fe

关 键 词：Bromoform metabolism Dibromomethane metabolism Methane mitigation METHANOGENS Rumen microbiota SEAWEED 

分 类 号：S816.3[农业科学—饲料科学]