两个棉蚜胰岛素受体生物信息学分析及其在三种有翅型棉蚜中的表达模式比较  

Bioinformatics analysis of two insulin receptors in Aphis gossypii and comparison of their expression patterns in three winged phenotypes

作　　者：王柳玉 王丽[2] 朱香镇 张开心 李东阳 姬继超[2] 崔金杰[2] 马伟华[1] Wang Liuyu;Wang Li;Zhu Xiangzhen;Zhang Kaixin;Li Dongyang;Ji Jichao;Cui Jinjie;Ma Weihua(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Institute of Cotton Research,Chinese Academy of Agricultural Sciences/National Key Laboratory of Cotton Bio-breeding and Integrated Utilization,Anyang,Henan 455000,China)

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室,河南安阳455000

出　　处：《棉花学报》2023年第6期497-508,共12页Cotton Science

基　　金：国家自然科学基金(32102214)。

摘　　要：【目的】明确棉蚜2个胰岛素受体基因的序列结构及其在3种有翅型棉蚜翅型分化与发育过程中的表达模式。【方法】基于棉蚜全基因组数据获取2个胰岛素受体基因(insulin receptor 1,AgInR1和insulin receptor 2,AgInR2)序列并进行氨基酸序列分析、系统发育分析,而后对InR1和InR2进行蛋白保守结构域分析以及基序分析。通过实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction,qRT-PCR)技术分析2种胰岛素受体基因在棉蚜孤雌无翅蚜以及3种有翅蚜不同发育时期的表达模式。【结果】理化性质分析结果显示AgInR1和AgInR2均为亲水性跨膜蛋白。系统发育分析结果显示17种昆虫的InR1聚为1簇,14种昆虫的InR2聚为1簇。半翅目蚜科的InR1、InR2分别以极高的自展值聚为一支,亲缘关系较近。保守结构域和基序分析表明棉蚜InR1和InR2保守结构排列较为相似,但相较于AgInR1,AgInR2缺少1个FN3结构域。qRT-PCR结果显示,AgInR1在孤雌有翅蚜、性母、雄蚜这3种有翅蚜的4龄、成虫期的表达量均显著高于同龄期的孤雌无翅蚜,而AgInR2在3种有翅蚜中的表达模式与AgInR1不同。【结论】AgInR1可能参与调控3种有翅蚜在4龄及成虫期的翅发育过程。AgInR2与AgInR1的表达模式不同,可能具有不同的生物学功能。该研究为进一步探究胰岛素信号通路在棉蚜翅型分化中的作用提供理论支持。[Objective]This study aims to clarify the sequence structure and expression pattern of two insulin receptor genes in three kinds of winged aphids of Aphis gossypii.[Methods]Based on the whole genome data of A.gossypii,amino acid sequence analysis of two insulin receptor genes(insulin receptor 1,AgInR1;insulin receptor 2,AgInR2)was identified,and phylogenetic analysis of insect InR1 from 17 insects and InR2 from 14 insect was performed.The protein conserved structural domains of insulin receptors were analyzed and motif analysis was conducted using online software.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to analyze the expression patterns of AgInR1 and AgInR2 in wingless female and three kinds of winged aphids at different developmental stages.[Results]The physicochemical analysis showed that AgInR1 and AgInR2 were hydrophilic transmembrane proteins.Phylogenetic analysis showed that InR1 and InR2 of the analyzed insects were clustered into two distinctive groups.The InR1 and InR2 of Hemiptera Aphididae were clustered together with very high bootstrap values,showed close affinity.Conserved domains and motif analyses showed that conserved domain arrangement of InR1 and InR2 from A.gossypii were relatively similar.Compared with AgInR1,AgInR2 lacked one FN3 domain.The results of qRT-PCR showed that the expression level of AgInR1 in the 4th instar and adult stages of the three winged aphids,namely,parthenogenetic wing female,gynoparae,and male was significantly higher than that of parthenogenetic wingless female at the same instar.Meanwhile the expression pattern of AgInR2 in the three kinds of winged aphids was different from that of AgInR1.[Conclusion]AgInR1 might involve in regulating the wing development process in the 4th instar and adult stages of three winged aphids.AgInR2 had a different expression pattern from AgInR1 and might have different biological functions.The study provides theoretical support for further investigation of the role of insulin signaling pathway in wing differentia

关 键 词：棉蚜 翅多态性 胰岛素受体 表达模式 翅型分化 

分 类 号：S435.622.1[农业科学—农业昆虫与害虫防治]