免疫组库测序在实体器官移植中的应用  

Application of immune repertoire sequencing in solid organ transplantation

作　　者：梁厉飞 陈婷婷 杨橙 Liang Lifei;Chen Tingting;Yang Cheng(Department of Urology,Zhongshan Hospital,Fudan University,Shanghai Key Laboratory of Organ Transplantation,Shanghai 200032,China;不详)

机构地区：[1]复旦大学附属中山医院泌尿外科上海市器官移植重点实验室,上海200032 [2]复旦大学附属中山医院药剂科 [3]上海浦东复旦大学张江科技研究院

出　　处：《器官移植》2024年第2期207-213,共7页Organ Transplantation

基　　金：国家自然科学基金(82170765);上海市青年拔尖人才开发计划;上海市临床重点专科(shslczdzk05802)。

摘　　要：免疫组库一般指T细胞和B细胞的总称,它有着巨大的多样性,使免疫系统能够对多种抗原刺激做出反应。随着测序技术的发展,免疫组库测序可以从基因水平深入了解排斥反应发生时淋巴细胞克隆的变化,也为基于免疫组库测序的新型无创诊断技术的产生提供了可能。近年来,免疫组库测序在实体器官移植中的尝试不断增多,特别是在肾移植、肝移植、心脏移植以及移植后感染等领域。本文对以上领域中应用免疫组库测序的研究进行了综述,总结器官移植中免疫组库测序使用的现状和作为早期无创诊断排斥反应的新技术的潜力,以期为这项技术进一步发展并应用于临床提供借鉴。Immune repertoire is defined as the sum of T cells and B cells,which possesses high diversity and enables immune system to respond to various antigen stimuli.With the development of sequencing technique,immune repertoire sequencing can be utilized to deeply understand the changes of lymphocyte clones when rejection occurs at the gene level,and also provide the possibility for the emergence of novel non-invasive diagnostic techniques based on immune repertoire sequencing.In recent years,more and more attempts have been made to apply immune repertoire sequencing in solid organ transplantation,especially in the fields of kidney transplantation,liver transplantation,heart transplantation and post-transplantation infection.In this article,research progresses on the application of immune repertoire sequencing in these fields were reviewed,and current status of immune repertoire sequencing in organ transplantation and its potential as a novel technique for early non-invasive diagnosis of rejection were summarized,aiming to provide reference for subsequent development and clinical application of this technique.

关 键 词：免疫组库 T细胞受体 B细胞受体 器官移植 排斥反应 感染 供者来源性细胞游离DNA(dd-cfDNA) T细胞介导的排斥反应(TCMR) 抗体介导的排斥反应(AMR) 供者特异性抗体(DSA) 程序性细胞死亡蛋白1 

分 类 号：R617[医药卫生—外科学] R392[医药卫生—临床医学]