孕康口服液通过TGF-β/Smads信号通路调节产后肾虚小鼠的复旧作用分析  

Yunkang Oral Liquid Promotes Restoration of Mice from Postpartum Kidney Deficiency via TGF-β/Smads Signaling Pathway

作　　者：郑佳亿 苏洁[1] 颜美秋[1] 俞静静[1] 陈奕公 傅萌 胡雪灵 常梦茜 吕圭源[1] 蒋立勤[1] ZHENG Jiayi;SU Jie;YAN Meiqiu;YU Jingjing;CHEN Yigong;FU Meng;HU Xueling;CHANG Mengxi;LYU Guiyuan;JIANG Liqin(Zhejiang Chinese Medical University,Hangzhou 311402,China)

机构地区：[1]浙江中医药大学,杭州311402

出　　处：《中国实验方剂学杂志》2024年第6期83-90,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：浙江省省级重点实验室项目(No.2012E10002)。

摘　　要：目的:探究孕康口服液对产后肾虚小鼠的作用。方法:将妊娠后小鼠随机分为4组,即模型组、孕康低剂量组(6 mL·kg^(-1))、孕康中剂量组(9 mL·kg^(-1))、孕康高剂量组(12 mL·kg^(-1)),睡眠剥夺与强迫游泳复合造模制备产后肾虚小鼠模型,造模同时灌胃给药,另设9只雌性ICR小鼠为正常组。检测小鼠动情周期、自主活动、背温、抓力、骨强度、舌苔色;酶联免疫吸附测定法(ELISA)检测血清雌二醇(E_(2))、孕酮(P)、促卵泡生长激素(FSH)和促黄体生成素(LH)水平;苏木素-伊红(HE)染色观察卵巢、子宫组织形态学改变;免疫组化和蛋白免疫印迹法(Western blot)检测卵巢和子宫转化生长因子(TGF)-β、Smad2/3蛋白表达。结果:模型组小鼠呈现动情周期延长,自主活动、背温、抓力、骨强度降低,小鼠舌薄白等症状。孕康口服液能缩短模型小鼠动情周期;提高自主活动、背温、抓力、骨强度;改善舌苔薄白;升高血清E_(2)、P水平,降低血清FSH、LH水平;降低卵巢卵泡闭锁率,促进子宫修复;降低卵巢及子宫组织TGF-β、Smad2/3蛋白表达。结论:孕康口服液对小鼠产后肾虚具有改善作用,其作用机制可能与调节TGF-β/Smads信号通路有关。Objective:To investigate the effect of Yunkang oral liquid on postpartum kidney deficiency in mice.Method:Postpartum mice were randomized into model and low-dose(6 mL·kg^(-1)),medium-dose(9 mL·kg^(-1)),and high-dose(12 mL·kg^(-1))Yunkang oral liquid groups.The mouse model of postpartum kidney deficiency was established by sleep deprivation combined with forced swimming.Another 9 female ICR mice were selected as the normal control group.The mice were administrated with Yunkang oral liquid during the period of modeling.The levels of estradiol(E_(2)),progesterone(P),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)in the serum were measured by the enzyme-linked immunosorbent assay.The morphological changes of ovaries and uterus were observed by hematoxylin-eosin(HE)staining,and the expression of transforming growth factor(TGF)-β and Smad2/3 was determined by immunohistochemistry and Western blotting.Result:The mice in the model group showed prolonged estrous cycle,reduced voluntary activity,dorsal temperature,grip strength,and bone strength,and whitening tongue coating.Compared with the model group,Yunkang oral liquid shortened the estrous cycle,increased the voluntary activity,dorsal temperature,grip strength,and bone strength,and alleviated the whitening of tongue coating.Moreover,it elevated the E_(2) and P levels and lowered the FSH and LH levels in the serum,decreased ovarian follicular atresia rate,promoted uterine repair,and down-regulated the expression of TGF-β and Smad2/3 in the ovarian and uterine tissues.Conclusion:Yunkang oral liquid can ameliorate postpartum kidney deficiency in mice by regulating the TGF-β/Smads signaling pathway.

关 键 词：孕康口服液 产后小鼠 肾虚 机制 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学] R289R287R22R2-031R33R24