石质土生境银柴胡根系不同层次真菌多样性与土壤性质、药材主要成分的关系  

Relationship Among Fungal Diversity at Different Levels of Root System of Stellaria dichotoma var.lanceolata,Soil Properties,and Main Components of Medicinal Materials in Lithosol Habitats

作　　者：刘王锁 邱开阳[3] 巴达日夫 高学花 杨智明 高敏 张芳 张波 LIU Wangsuo;QIU Kaiyang;BA Darifu;GAO Xuehua;YANG Zhiming;GAO Min;ZHANG Fang;ZHANG Bo(Department of Chemical and Environmental Engineering,Hetao College,Bayannur 015000,China;Ningxia Technical College of Wine and Desertification Prevention,Yinchuan 750199,China;School of Forestry and Prataculture,Ningxia University,Yinchuan 750021,China;School of Resources Environment and Life Sciences,Ningxia Normal University,Guyuan 756000,China;Ningxia Forest Pest Control and Quarantine Station,Yinchuan 750001,China)

机构地区：[1]河套学院化学与环境工程系,内蒙古巴彦淖尔015000 [2]宁夏葡萄酒与防沙治沙职业技术学院,银川750199 [3]宁夏大学林业与草业学院,银川750021 [4]宁夏师范学院资源环境与生命科学学院,宁夏固原756000 [5]宁夏森林病虫防治检疫总站,银川750001

出　　处：《中国实验方剂学杂志》2024年第6期160-168,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：宁夏自然科学基金项目(2022AAC03641)。

摘　　要：目的:探究石质土生境下银柴胡根系真菌与土壤因子及其药材主要成分之间的互作关系。方法:采用高通量测序技术,测定银柴胡根系不同层次(根围、根际、根表和根内)真菌群落与根系(根际与根围)土壤性质,分析真菌群落与土壤性质、药材主要成分的关系。结果:银柴胡根际土壤全磷、速效磷、碱解氮、可溶性有机碳及水分含量略高于根围,但差异不显著。银柴胡根系真菌优势门以子囊菌门(Ascomycota)为主,在根围-根际-根表-根内根系层次中,真菌多样性虽逐渐降低,但肉座菌目(Hypocreales)、可能新门unclassified_k_Fungi、柔膜菌目(Helotiales)及那提普斯壳目(Natipusillales)的成员丰度逐渐增加,其中肉座菌目是银柴胡根系最主要的真菌类群。结构方程模型(SEM)表明,根系土壤理化因子对真菌群落和药材主要成分起到了重要的调节作用,其中土壤全氮、碱解氮、水分和pH是主要的调节因子,土壤氮素含量是促进药材主要成分的关键,青霉属(Penicillium)真菌也是调节药材主要成分的关键真菌类群。结论:银柴胡土壤理化性质对真菌群落和药材成分具有重要的作用,其根系肉座菌目真菌是较为重要的类群,而青霉菌属真菌对药材成分具有一定的介导作用。Objective:To explore the interaction among root fungi of Stellaria dichotoma var.lanceolata,soil factors,and main components of medicinal materials in lithosol habitats.Method:Highthroughput sequencing technology was employed to determine the fungal community of the root system of S.dichotoma var.lanceolata at different levels(bulk soil,rhizosphere soil,rhizoplane soil,and root interior)and the soil properties of the root system(bulk and rhizosphere),and the relationship among the fungal community,soil properties,and the main components of medicinal materials was analyzed.Result:The total phosphorus,available phosphorus,alkaline nitrogen,dissolved organic carbon,and soil water content in the rhizosphere soil of S.dichotoma var.lanceolata were slightly higher than those in the rhizosphere,but the difference was not significant.Ascomycota is the dominant phylum of root fungi in S.dichotoma var.lanceolata.In the progressive level of bulk-rhizosphere-rhizoplane-root interior system,although the fungal diversity gradually decreased,the abundance of Hypocreales,a new phylum(unclassified_k_Fungi),Helotiales,and Natipusilales gradually increased,among which Hypocreales is the most important fungal group in the root system of S.dichotoma var.lanceolata.The structural equation model(SEM)shows that the physicochemical factors of the root-soil play an important regulatory role in the fungal community and the main components of medicinal herbs,with soil total nitrogen,alkaline nitrogen,soil water content,and pH being the main regulatory factors.Soil nitrogen content is the key to promoting the main components of the medicinal herbs,and Penicillium fungi are the key fungal group to regulate the main components of the medicinal herbs.Conclusion:It highlights that the physicochemical properties of the soil of S.dichotoma var.lanceolata play a crucial role in the fungal community and the components of medicinal materials.Hypocreales fungi in the root of S.dichotoma var.lanceolata were an important group,and Penicillium fungi had

关 键 词：银柴胡 真菌 石质土 土壤性质 药材成分 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学] R287R22R2-031R33R24