高寒湿地植物对水淹胁迫的生理响应及适应性评价  

Physiological Response and Adaptation Evaluation of Alpine Wetland Plants to Waterlogging Stress

作　　者：湛宇 李长慧[1] 才华 肖锋 季海川 马元杰 周浪敏 杨明春 ZHAN Yu;LI Chang-hui;CAI Hua;XIAO Feng;JI Hai-chuan;MA Yuan-jie;ZHOU Lang-min;YANG Ming-chun(Qinghai University,Xining,Qinghai Province 810016,China;Zequ Forest Farm of Zeku County of Qinghai Province,Huangnan,Qinghai Province 811400,China;Qinghai Province Wetland Protection Center,Xining,Qinghai Province 810008,China)

机构地区：[1]青海大学,青海西宁810016 [2]泽曲林场,青海黄南811400 [3]青海省湿地保护中心,青海西宁810008

出　　处：《草地学报》2024年第2期517-526,共10页Acta Agrestia Sinica

基　　金：湿地湿生植物适应性研究(2022-ZQ-1)资助。

摘　　要：为探究高寒湿地植物的耐水淹机理,测定杉叶藻(Hippuris vulgaris)等5种高寒湿地植物水淹40 d后的形态和生理生化指标,利用耐水淹系数,采用PCA分析、相关性分析和隶属函数法构建水淹胁迫适应性评价体系。结果表明:水淹胁迫下5种植物抗氧化酶活性上升1~4倍,以抵抗水淹胁迫,适应水淹环境;不同植物地上部分和地下部分丙二醛含量、抗氧化酶活性和渗透调节物质对水淹胁迫的响应有所不同;通过PCA分析和相关性分析,株高、光合色素含量、SS含量、SP含量、地上部分POD活性和地上部分SOD活性可作为高寒湿地植物水淹胁迫适应性筛选的主要评价指标;对综合指标(PC1~4)进行加权和隶属函数分析,5种高寒湿地植物水淹胁迫适应性由强到弱顺序为杉叶藻>三裂碱毛茛>光稃早熟禾>双柱头蔺藨草>中国马先蒿。To explore the mechanism of waterlogging tolerance in alpine wetland plants,the morphological,physiological,and biochemical indexes of 5 plants,including Hippuris vulgaris were determined after 40 days of waterlogging.The evaluation system of waterlogging stress adaptability was constructed by PCA analysis,correlation analysis,and membership function method using waterlogging tolerance coefficient.The results showed that the activity of antioxidant enzymes increased by 1~4 times under waterlogging stress to resist waterlogging stress and adapt to a waterlogging environment.The responses of malondialdehyde content,antioxidant enzyme activity,and osmoregulatory substances to waterlogging stress were different in the shoot and root parts of different plants.Through PCA analysis and correlation analysis,plant height,photosynthetic pigment content,soluble sugar content,soluble protein content,peroxidase activity,and superoxide dismutase activity in the shoot can be used as the main evaluation indexes for the adaptability screening of plants under waterlogging stress in alpine wetland.According to the weighted and membership function analysis of the comprehensive indexes(PC1~4),the order of adaptability to waterlogging stress of the 5 alpine wetland plants was Hippuris vulgaris>Halerpestes tricuspis>Poa araratica subsp.Psilolepis>Trichophorum distigmaticum>Pedicularis chinensis.

关 键 词：高寒湿地植物 水淹 生理生化 隶属函数 适应性评价 

分 类 号：Q945.78[生物学—植物学]