基于mtDNA COⅠ基因对榕属植物上粉蚧的分子鉴定和系统发育分析  

Molecular identification and phylogenetic analysis of mealybug species in Ficus spp.based on mitochondrial COⅠ gene

作　　者：傅卫民 刘志红 蔡波 李惠萍 吴福中 FU Weiming;LIU Zhihong;CAI Bo;LI Huiping;WU Fuzhong(City Polytechnic of Shenzhen,Shenzhen,Guangdong 518116,China;Shenzhen Institute of Technology,Shenzhen,Guangdong 518116,China;Agricultural Technology Promotion Center at Huicheng District Huizhou City,Huizhou,Guangdong 516001,China;Post-Entry Quarantine Station for Tropical Plant,Haikou Customs District,Haikou,Hainan 570105,China;Technology Center of Taiyuan Customs District,Taiyuan,Shanxi 030006,China;Technology Center of Huizhou Customs House,Huizhou,Guangdong 516006,China)

机构地区：[1]深圳城市职业学院,广东深圳518116 [2]深圳技师学院,广东深圳518116 [3]惠州市惠城区农业技术推广中心,广东惠州516001 [4]海口海关热带植物隔离检疫中心,海南海口570105 [5]太原海关技术中心,山西太原030006 [6]惠州海关综合技术中心,广东惠州516006

出　　处：《生物安全学报（中英文）》2024年第1期12-18,共7页Journal of biosafety

基　　金：广东省科技项目(2019A141401001);广东省科技奖入库培育项目(2021C0405004);海南省自然科学基金(321RC666);惠州市科技项目(2017C0424040);深圳技师学院科研项目(2031001、2314001)。

摘　　要：【目的】榕属植物是我国华南地区普遍种植的园林植物,本研究旨在明确榕属植物上发生危害的粉蚧种类。【方法】测定榕属植物上发生为害的9种粉蚧的COⅠ基因序列,利用生物信息学软件分析其序列同源性、遗传结构及系统进化关系。【结果】测出38条粉蚧的COⅠ基因序列相似度达到99.25%~100.00%,9种粉蚧的种间遗传距离为0.070~0.173,其中堆蜡粉蚧与菠萝灰粉蚧遗传距离最大,而康氏粉蚧与橘小粉蚧遗传距离最小,说明康氏粉蚧与橘小粉蚧亲缘关系较近。构建的系统发育树中,每种粉蚧与GenB ank数据库下载的已知序列聚在一支,系统发育树结果与形态学鉴定结果一致。【结论】线粒体COⅠ基因序列能够快速准确鉴定榕属植物上的粉蚧,也揭示了榕属植物上粉蚧的遗传结构和系统进化关系,为农林业监测和精准防治粉蚧类害虫提供科学依据。【Aim】Ficus spp.are commonly planted garden plants in the South China.The aim is to identify the species of mealybugs in Ficus spp.【Method】The COⅠgene sequences of 9 species of mealybugs in Ficus spp.were amplified,and their sequence homology,genetic structure,and phylogenetic relationship were analyzed using bioinformatics software.【Result】The similarity of 38 sequences of mitochondrial COⅠ gene reached 99.25%-100.00%,and the interspecific genetic distance of 9 mealybugs was 0.070-0.173.Nipaecoccus viridis and Dysmicoccus brevipes had the highest genetic distance,while Pseudococcus comstocki and Pseudococcus cryptus was the smallest,which indicated that Pseudococcus comstocki and Pseudococcus cryptus were highly closely related.The phylogenetic tree was constructed with each mealybug clustered together using known mealybug sequences downloaded separately from the GenBank database.The results of molecular identification were found to be the same as those of morphological identification.【Conclusion】The mitochondrial COⅠ gene could rapidly and accurately identify mealybugs in Ficus spp.and reveal the genetic structure and phylogenetic relationship of mealybugs.It provided a scientific basis for the monitoring,prevention,and precise control of mealybugs in agriculture and forestry.

关 键 词：榕属植物 粉蚧 分子鉴定 线粒体COⅠ基因 系统发育 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]