4种烟碱受体激动剂对松材线虫的毒杀活性  

The activity of four nicotinic receptor agonists against Bursaphelenchus xylophilus

作　　者：于金英 吕耀平[1] 王国丽 刘冬梅 雷秋艳 贺小如 汪冶[1] YU Jinying;L Yaoping;WANG Guoli;LIU Dongmei;LEI Qiuyan;HE Xiaoru;WANG Ye(College of Ecology,Lishui University,Lishui,Zhejiang 323000,China;College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou,Zhejiang 310002,China)

机构地区：[1]丽水学院生态学院,浙江丽水323000 [2]浙江理工大学生命科学与医药学院,浙江杭州3100002

出　　处：《生物安全学报（中英文）》2024年第1期48-53,67,共7页Journal of biosafety

基　　金：国家自然科学基金项目(31801306);浙江省自然科学基金项目(Y3110477)。

摘　　要：【目的】探究4种烟碱受体激动剂对松材线虫的杀虫作用。【方法】采用浸渍法以及马尾松松枝水培试验分别进行杀线虫活性测定,并测定4种烟碱受体激动剂水溶液对松材线虫体内三磷酸腺苷酶(ATP)、过氧化氢酶(CAT)、乙酰胆碱酯酶(AChE)、超氧化物歧化酶(SOD)的影响。【结果】盐酸左旋咪唑、盐酸四咪唑、烟碱、酒石酸噻吩嘧啶4种烟碱受体激动剂对松材线虫有良好的毒杀活性,4种药物水溶液浸渍处理松材线虫48 h,半致死浓度LC_(50)值分别为0.09、0.31、0.08、0.14 mg·mL^(-1);马尾松水培试验处理12 d后结果显示,盐酸左旋咪唑、盐酸四咪唑、烟碱、CK处理组松枝均不发病,病情指数为0,酒石酸噻吩嘧啶处理组部分枝叶变黄,病情指数为31%,“CK+线虫”松枝枝叶完全变黄萎蔫,病情指数为100%;4种烟碱受体激动剂水溶液浸渍处理48 h后松材线虫体内ATP、CAT、AChE、SOD酶活性均低于对照组(P<0.05)。【结论】盐酸左旋咪唑、盐酸四咪唑、烟碱、酒石酸噻吩嘧啶对松材线虫有良好的防治潜力,具有进一步深入研究和开发利用的前景。【Aim】To investigate the insecticidal effect of four nicotine receptor agonists against pine wood nematode,Bursapherenchus xylophilus.【Method】The nematicidal activity was measured on pine branches of Pinus massoniana grown in hydroponics.The effects of nicotinic receptor agonists in aqueous solution were examined on the activities of adenosine triphosphate(ATP),catalase(CAT),acetylcholinesterase(AChE),and superoxide dismutase(SOD)in B.xylophilus.【Result】Levamisole hydrochloride(LH),tetraimidazole hydrochloride(TH),nicotine(N),and thiophene pyrimidine tartrate(TPT)had significant toxic activity against B.xylophilus.The LC_(50) values of the four drugs were 0.09,0.31,0.08,and 0.14 mg·mL^(-1),respectively,when immersed in water for 48 h.The results of the hydroponic experiments after 12 d showed that the pine branches in the LH,TH,N,and CK treatment groups did not suffer from the disease(index-0).Some branches and leaves in the TPT treatment group turned yellow,with a disease index of 31%.The branches and leaves of"CK+nematode"pine completely turned yellow and wilted,with a disease index of 100%.ATP,CAT,AChE,and SOD activities in B.xylophilus treated with nicotinic receptor agonists(LH,TH,N,and TPT)for 48 h were lower than those in the control group(P<0.05).【Conclusion】In conclusion,nicotinic receptor agonists(LH,TH,N,and TPT)have potential as good biological controls against B.xylophilus,and have the prospect of further in-depth research,development,and utilization.

关 键 词：盐酸左旋咪唑 盐酸四咪唑 烟碱 酒石酸噻吩嘧啶 松材线虫 杀线活性 酶活性 

分 类 号：S763.18[农业科学—森林保护学]