蚕豆象的线粒体基因组研究及系统发育分析  

The mitochondrial genome and phylogenetic analysis of Bruchus rufimanus(Boheman,1833)

作　　者：林兴雨 宋南[1] LIN Xingyu;SONG Nan(College of Plant Protection,Henan Agricultural University,Zhengzhou,Henan 450046,China)

机构地区：[1]河南农业大学植物保护学院,河南郑州450046

出　　处：《生物安全学报（中英文）》2024年第1期83-92,共10页Journal of biosafety

基　　金：国家自然科学基金项目(U1904104)。

摘　　要：【目的】蚕豆象是农业上一种重要害虫,本文测序、分析了蚕豆象的线粒体全基因组,以期为更好地理解茎甲亚科分支类群、豆象亚科和蚕豆象的系统发育提供线粒体基因组数据。【方法】在系统发育分析中,内群取样包含茎甲亚科分支类群的22种昆虫,外群选择2个肖叶甲亚科(Basilepta fulvipes和Basilepta melanopus)昆虫,分别利用最大似然法和贝叶斯法重建茎甲亚科分支类群的系统发育关系。【结果】蚕豆象的线粒体基因组全长为16586 bp(GenB ank序列号:OP650255),包含37个基因(13个蛋白质编码基因、2个核糖体RNA基因和22个转运RNA基因)和一段非编码控制区。所有转运RNA基因中,除trnS1因缺少二氢尿嘧啶(DHU)臂而形成一个简单的环,无法构成稳定的三叶草结构外,其余转运RNA基因均能形成典型的三叶草结构。此外,trnS1的反密码子不是常见的GCU,而是UCU。【结论】本研究首次获得了蚕豆象的线粒体基因组全序列。2种不同的系统发育分析方法构建的系统发育关系显示:豆象亚科、茎甲亚科、水叶甲亚科和负泥虫亚科为单系群;豆象亚科与水叶甲亚科+负泥虫亚科为姐妹群;蚕豆象与四纹豆象为姐妹群。【Aim】The Bruchus rufimanus is an important agricultural insect pest.In this study,we sequenced and analyzed the mitochondrial genome of B.rufimanus,and it will contribute to increasing our knowledge of the mitochondrial genome and the phylogeny of"Sagrinae"clade group,Bruchinae and B.rufimanus.【Method】In phylogenetic analyses,we selected 22 exemplars of"Sagrinae"clade group(Bruchinae,Sagrinae,Donaciinae and Criocerinae)as ingroups.Two species of Eumolpinae(Basilepta fulvipes and Basilepta melanopus)were used as outgroups.The phylogenetic analyses were conducted using maximum likelihood and Bayesian inference methods.【Result】The mitochondrial genome of B.rufimanus is a circular molecule of 16586 bp in length(GenBank accession No.OP650255),which contains 13 protein-coding genes,two ribosomal RNA genes,22 transfer RNA genes and a non-coding control region.All tRNA genes can be folded into typical cloverleaf structure,with the exception of the trnS1,which lacks the DHU arm.Furthermore,the anticodon of trnS1 gene is not the common GCU,but rather the UCU.【Conclusion】This is the first report of a complete mitochondrial genome of B.rufimanus.Both phylogenetic inference methods produced a similar tree topological structure:the subfamily Bruchinae,Sagrinae,Donaciinae and Criocerinae were recovered the monophyletic groups.The subfamily Bruchinae were cluster to(Donaciinae+Criocerinae)a clade.The B.rufimanus was sister to Callosobruchus maculatus.

关 键 词：鞘翅目 豆象亚科 蚕豆象 线粒体基因组 系统发育 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]