IL-4、IL-13在大疱性类天疱疮患者血清和疱液中的表达  

The expression levels of IL-4 and IL-13 in the serum and blister fluid of patients with bullous pemphigoid

作　　者：周瑶 滕理君 李帼敏 徐洁 郑金津 房浩 薛汝增 ZHOU Yao;TENG Lijun;LI Guomin;XU Jie;ZHENG Jinjin;FANG Hao;XUE Ruzeng(Dermatology Hospital,Southern Medical University,Guangzhou 510091,China)

机构地区：[1]南方医科大学皮肤病医院,广东广州510091

出　　处：《皮肤性病诊疗学杂志》2024年第1期1-8,共8页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：南方医科大学皮肤病医院临床研究项目(B2019001)。

摘　　要：目的探讨2型炎症因子IL-4、IL-13在大疱性类天疱疮(BP)患者血清和疱液中的表达情况。方法收集BP患者(49例)及对照组(健康人,33例)的血清或疱液样本,通过ELISA法检测样本中IL-4、IL-13的水平,比较两组血清和疱液样本中IL-4、IL-13水平是否存在差异;分析BP患者血清和疱液样本中IL-4、IL-13水平与血清总IgE水平、外周血嗜酸性粒细胞水平、荨麻疹样红斑评分、瘙痒评分之间的关系;分析抗BP180、BP230抗体谱的不同表达与IL-4、IL-13、血清总IgE及外周血嗜酸性粒细胞水平之间的相关性。结果BP患者血清中IL-4、IL-13的水平与对照组相比均无明显差异(均P>0.05),而疱液中IL-4、IL-13的表达均较对照组明显升高(P<0.01)。IL-4、IL-13在BP患者血清中的表达与在疱液中的表达无相关性。BP患者血清中IL-4、IL-13水平与血清总IgE水平、外周血嗜酸性粒细胞水平、荨麻疹样红斑评分、瘙痒评分均无明显相关性,而疱液中IL-4的表达与血清IgE水平呈正相关(r=0.98,P=0.021)。BP患者血清抗体谱单阳性(抗BP180抗体阳性,抗BP230抗体阴性)的表达与疱液中IL-13表达水平呈正相关(r=0.58,P=0.009)。结论IL-4、IL-13在BP皮肤局部富集,提示BP存在局部2型炎症。BP患者血清总IgE水平可能反映局部2型炎症反应的严重程度。BP180免疫复合物可能通过局部IL-13的募集影响2型炎症反应的发生过程,或可有助于未来通过血清抗体谱进行分层精准靶向治疗。Objective To investigate the expression levels of type 2 inflammatory cytokines,IL-4 and IL-13,in the serum and blister fluid of patients with bullous pemphigoid(BP).Methods The serum or blister fluid was collected from BP patients(49 cases)and healthy individuals(33 cases).ELISA was used to measure the concentrations of IL-4 and IL-13.The expression levels of IL-4 and IL-13 were compared between the BP patients and healthy controls.The correlations of IL-4 and IL-13 with total serum IgE levels,eosinophils,skin erythema score and pruritus severity(NRS)in BP patients were analyzed.Moreover,the associations of BP180 and BP230 IgG antibodies with IL-4,IL-13 and total serum IgE levels,and eosinophils were determined.Results The concentrations of IL-4 and IL-13 in the blister fluid,but not in the serum,were significantly higher in the BP patients than in the healthy controls(P<0.01).Serum levels of either IL-4 or IL-13 were not significantly correlated with total serum IgE levels,eosinophils,erythema score and NRS in BP patients.However,IL-4 levels in blister fluid were positively correlated with total serum IgE levels in BP patients(r=0.98,P=0.021).The IL-13 levels in blister fluid were associated with serum BP180 positive,but BP230 negative(r=0.58,P=0.009).Conclusions Elevated IL-4 and IL-13 levels in the involved skin site of bullous pemphigoid suggest the present of local type 2 inflammation.Total IgE levels may reflect the severity of local type 2 inflammation.BP180 complex may affect the development of type 2 inflammation through increasing local IL-13 levels.These findings might be helpful in the target therapy in the future.

关 键 词：大疱性类天疱疮 2型炎症 IL-4 IL-13 嗜酸性粒细胞 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]