几种战创伤止血敷料的抑菌性能研究  

Study on bacteriostatic activity of several hemostatic dressings for war injury

作　　者：张丽红 李素哲 高明 路彦茹 楚雪欣 张志宝 董科 ZHANG Li-hong;LI Su-zhe;GAO Ming;LU Yan-ru;CHU Xue-xin;ZHANG Zhi-bao;DONG Ke(Shijiazhuang Yishengtang Medical Product Co.,Ltd.,Shijiazhuang 050200,China)

机构地区：[1]石家庄亿生堂医用品有限公司,石家庄050200

出　　处：《医疗卫生装备》2023年第12期31-35,共5页Chinese Medical Equipment Journal

基　　金：军队科研项目(BWS19J007);河北省军民两用关键技术和产品研发专项(SJMYF2022X14)。

摘　　要：目的:研究5种战创伤止血敷料的抑菌性能,以为研制新型战创伤止血敷料提供参考。方法:依据GB/T 20944.2—2007检测Combat Gauze(成分为高岭土)及Celox Rapid Gauze、Celox Gauze、ChitoGauze、自制敷料(成分均为壳聚糖)对金黄色葡萄球菌、大肠杆菌的抑菌率,并通过分析模拟条件下金黄色葡萄球菌的生长情况来推断止血敷料的抑菌时长及抑菌效果。结果:Combat Gauze在24 h内对金黄色葡萄球菌和大肠杆菌的抑菌率均低于20%。Celox Rapid Gauze、Celox Gauze、ChitoGauze和自制敷料4种止血敷料对金黄色葡萄球菌作用30 min时抑菌率均超过90%,但对大肠杆菌的抑菌率略有不同,且随着作用时间的延长而变化:作用30 min时,只有Celox Rapid Gauze的抑菌率大于90%;作用3 h时,ChitoGauze的抑菌性相对较弱,仅为35%,其他3种敷料的抑菌率均大于95%;作用24 h时,4种敷料的抑菌率均大于99%。Celox Rapid Gauze、Celox Gauze、ChitoGauze和自制敷料在15 h内均可显著抑制金黄色葡萄球菌的生长,且在模拟使用条件下,金黄色葡萄球菌达到临床感染临界值的时间分别为18、15、24和15 h。结论:Combat Gauze对金黄色葡萄球菌和大肠杆菌无抑菌作用,Celox Rapid Gauze、Celox Gauze、ChitoGauze和自制敷料4种止血敷料具有较好的抑菌性能,且Celox Rapid Gauze的综合抑菌性能较好,ChitoGauze对金黄色葡萄球菌的抑菌时间最长。Objective To investigate the bacteriostatic activity of five hemostatic dressings for war injury to provide references for the development of novel hemostatic dressings.Methods The bacteriostatic ratios of Combat Gauze made of kaolin and four kinds of dressings made of chitosan including Celox Rapid Gauze,Celox Gauze,ChitoGauze and a self-developed dressing against S.aureus and E.coli were explored according to GB/T 20944.2—2007.The bacteriostatic time and activity were inferred by investigating the growth of S.aureus under simulated conditions.Results Combat Gauze had the hemostatic ratios lower than 20%against both S.aureus and E.coli within 24 h.The hemostatic ratios of Celox Rapid Gauze,Celox Gauze,ChitoGauze and the self-developed dressing against S.aureus were all higher than 90%after 30 min action,while the ratios of the four dressings against E.coli were slightly different and changed with the prolongation of the time of action:after 30 min action only Celox Rapid Gauze had the hemostatic ratio higher than 90%;after 3 h action,ChitoGauze had a low ratio of 35%while the other dressings were all higher than 95%;after 24 h action the four dressings all had the ratios higher than 99%.Celox Rapid Gauze,Celox Gauze,ChitoGauze and the self-developed dressing all significantly inhibited the growth of S.aureus within 15 h and the time for S.aureus to reach the threshold of clinical infection under simulated conditions was 18,15,24 and 15 h,respectively.Conclusion Combat Gauze is not effective in inhibiting S.aureus and E.coli,while Celox Rapid Gauze,Celox Gauze,ChitoGauze and the self-developed dressing behave well with Celox Rapid Gauze gaining high compre-hensive bacteriostatic activity and ChitoGauze having the longest bacteriostatic time against S.aureus.

关 键 词：战创伤 止血敷料 抑菌性能 壳聚糖 高岭土 金黄色葡萄球菌 大肠杆菌 

分 类 号：R318[医药卫生—生物医学工程]