Bone analysis using an aggregation-induced emission-active iridium complex  

作　　者：Xianpeng Zhang Xinling Liu Hongwen Yu Shuang Shen Junchao Zhi Zhen GaoJingqi Xin Jiaqi Song Liang Shao Caiting Meng Feifei An Taotao Huo Shichang Liu Ye Zhang Li Xu Guanying Li 

机构地区：[1]Department of Biophysics,School of Basic Medical Sciences,Health Science Centre,Xi’an Jiaotong University,Xi’an,P.R.China [2]School of Chemistry and Chemical Engineering,Guangdong Pharmaceutical University,Zhongshan,P.R.China [3]Department of Spine Surgery,Honghui Hospital,Xi’an Jiaotong University,Xi’an,P.R.China [4]Active Soft Matter Group,Songshan Lake Materials Laboratory,Dongguan,P.R.China [5]School of Public Health,Health Science Centre,Xi’an Jiaotong University,Xi’an,P.R.China

出　　处：《Aggregate》2023年第6期110-119,共10页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Number:22107087;Yong Talent Support Plan of Xi’an Jiaotong University,Grant/Award Number:YX6J024;Science and Technology Planning Project of Guangzhou,Grant/Award Number:202002030089;Key Projects of Social Welfare and Basic Research of Zhongshan City,Grant/Award Number:2021B2007。

摘　　要：Fluorescent analysis of bone provides valuable insights into bone structures.However,conventional dyes suffer from low specificity on bone tissue,small stokes shift,short fluorescent lifetime,and aggregation-caused quenching effect,which result in low efficacy and artifacts.In this work,we design an aggregation-induced emission(AIE)-active iridium(III)complex(Ir-BP2)as a highly selective,convenient,nondestructiveness,and dual-mode staining agent for bone analysis.Ir-BP2 containing phosphonate groups selectively binds to hydroxyapatites,the main component of bone matrix,and exhibits turn-on AIE phosphorescence with prolonged lifetime.Ir-BP2 exhibits promising biosafety and offers higher accuracy in staining calcium deposits than conventional Alizarin Red S staining assay when it is employed in real-time monitoring of osteogenesis differentiation process.A ready-to-use staining spray of Ir-BP2 is fabricated.By using fluorescent imaging and lifetime imaging,Ir-BP2 staining provides valuable insights into bone microstructure analysis,microdamage diagnosis,and bone growth state identification.Further,Ir-BP2 is successfully applied on a human spine vertebra for diagnosing bone invasiveness of eosinophilic granuloma,validating its clinical practice.This work presents a powerful tool in bone analysis and will lead to new approaches for the diagnosis and treatment of bone-related diseases.

关 键 词：aggregation-induced emission bone analysis clinical diagnosis eosinophilic granuloma iridium complexes phosphorescent probe 

分 类 号：O62[理学—有机化学]