Self-assembly multifunctional DNA tetrahedron for efficient elimination of antibiotic-resistant bacteria  

作　　者：Tiantian Wu Yu Fu Shuang Guo Yanqiang Shi Yuxin Zhang Zhijin Fan Bin Yang Baoquan Ding Yuhui Liao 

机构地区：[1]Molecular Diagnosis and Treatment Center for Infectious Diseases,Dermatology Hospital,Southern Medical University,Guangzhou,China [2]CAS Key Laboratory of Nanosystem and Hierarchical Fabrication,CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology,Beijing,China [3]Longgang District Central Hospital of Shenzhen,Shenzhen,China

出　　处：《Aggregate》2024年第1期204-215,共12页聚集体（英文）

基　　金：National Key R&D Program of China,Grant/Award Numbers:2021YFA1200302,2021YFC2302200,2018YFA0208900;National Natural Science Foundation of China,Grant/Award Numbers:22025201,22077023,82202532,82272248,82002244,81972019;Natural Science Fund of Guangdong Province for Distinguished Young Scholars,Grant/Award Number:2022B1515020089;China Postdoctoral Science Foundation,Grant/Award Numbers:2022M711528,2021M691428。

摘　　要：Antibiotic resistance is a major challenge in the clinical treatment of bacterial infectious diseases.Herein,we constructed a multifunctional DNA nanoplatform as a versatile carrier for bacteria-specific delivery of clinical antibiotic ciprofloxacin(CIP)and classic nanoantibiotic silver nanoparticles(AgNP).In our rational design,CIP was efficiently loaded in the self-assembly double-bundle DNA tetrahedron through intercalation with DNA duplex,and single-strand DNA-modified AgNP was embedded in the cavity of the DNA tetrahedron through hybridization.With the site-specific assembly of targeting aptamer in the well-defined DNA tetrahedron,the bacteria-specific dual-antibiotic delivery system exhibited excellent combined bactericidal properties.With enhanced antibiotic accumulation through breaking the out membrane of bacteria,the antibiotic delivery system effectively inhibited biofilm formation and promoted the healing of infected wounds in vivo.This DNAbased antibiotic delivery system provides a promising strategy for the treatment of antibiotic-resistant infections.

关 键 词：antibiotic resistance anti-infection therapy BIOFILM DNA nanotechnology targeted delivery 

分 类 号：R978.1[医药卫生—药品]